基于“肺与大肠相表里”理论探讨射干止咳胶囊对耐甲氧西林金黄色葡萄球菌感染肺炎大鼠肠道菌群的影响  

Effect of Shegan Zhike Capsule(射干止咳胶囊)on Intestinal Flora of Pneumonia Rats Infected with Methicillin-resistant Staphylococcus Aureus Based on Theory of“Lung and Large Intestine”

作　　者：时艳杰 张颖[2] 甘雨[2] 赵玥 尹江涛 梁丽喆 曲妮妮[1] 李国信[2] SHI Yanjie;ZHANG Ying;GAN Yu;ZHAO Yue;YIN Jiangtao;LIANG Lizhe;QU Nini;LI Guoxin(Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China;Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110034,Liaoning,China)

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032 [2]辽宁中医药大学附属第二医院,辽宁沈阳110034

出　　处：《中华中医药学刊》2024年第11期55-60,I0013-I0018,共12页Chinese Archives of Traditional Chinese Medicine

基　　金：国家科技部项目(2017ZX09301019);辽宁省科技厅重点研发计划项目(2020JH2/10300090);辽宁省科技厅博士启动基金项目(2022-BS-068)。

摘　　要：目的基于“肺与大肠相表里”的中医理论,应用16SrDNA分析射干止咳胶囊对耐甲氧西林金黄色葡萄球菌(MRSA)感染肺炎大鼠肠道菌群的影响,探讨射干止咳胶囊对大鼠MRSA肺炎的作用及机制。方法取60只SD大鼠随机分为6组,10只/组,分别为阴性对照组、模型对照组、射干止咳胶囊低、中、高剂量组和苏黄止咳胶囊组,除阴性对照组外,其余各组滴鼻感染MRSA连续5 d,造模期间同时灌胃给药,1次/d,连续14 d,阴性对照组、模型对照组给予等体积的纯水。末次给药前1 d进行粪便采集,放入-80℃保存,准备进行16SrDNA高通道测序。末次给药后1 h,对所有存活动物麻醉后进行血常规检测、肺组织病理切片。根据6组大鼠的病理报告和血常规结果,取阴性对照组、模型对照组、射干止咳胶囊低、中剂量组、苏黄止咳胶囊组各组6只大鼠粪便进行16SrDNA测序。结果通过16SrDNA测序,菌群多样性和丰富度方面,与阴性对照组比,虽然模型组有上调的趋势,但差异未有统计学意义;与模型组相比,射干止咳胶囊菌群的多样性和相对丰度有下调的趋势,并向阴性对照组接近,但是差异未有统计学意义。厚壁菌门、拟杆菌门、变形菌门为大鼠的优势菌门,与模型组相比,射干止咳胶囊分别上调了拟杆菌门、下调了变形菌门。通过菌群基因功能预测,与模型组相比,其中烟酸和烟酰胺代谢是射干止咳胶囊中、低剂量组共同作用的代谢通路。结论射干止咳胶囊可能通过上调了拟杆菌门、下调了变形菌门改善了MRSA肺炎大鼠的肠道内稳态,从烟酸和烟酰胺代谢通路发挥对MRSA肺炎大鼠的作用。Objective Based on the theory of“lung and large intestine”in traditional Chinese medicine,16SrDNA was used to analyze the effect of Shegan Zhike Capsule(射干止咳胶囊)on intestinal flora in rats with pneumonia caused by methicillin-resistant staphylococcus aureus,and to explore the effect and mechanism of Shegan Zhike Capsule on methicillin-resistant staphylococcus aureus(MRSA)pneumonia in rats.Methods Sixty SD rats were randomly divided into 6 groups,10 rats in each group,which were negative control group,model control group,low,medium and high dose groups of Shegan Zhike Capsule and Suhuang Zhike Capsule(苏黄止咳胶囊)group.Except the negative control group,the other groups were infected with MRSA by nasal drip for 5 consecutive days.During the modeling period,intragastric administration was given once a day for 14 consecutive days.The negative control group and the model control group were given the equal volume of pure water.The feces were collected the day before the last administration and stored at-80℃for 16SrDNA high-channel sequencing.One hour after the last administration,blood routine test and lung tissue pathological section were performed on all surviving animals after anesthesia.According to the pathological reports and blood routine results of 6 groups,the feces of 6 rats in negative control group,model control group,low-dose and middle-dose groups of Shegan Zhike Capsule and Suhuang Zhike Capsule group were taken for 16SrDNA sequencing.Results By 16SrDNA sequencing,compared with those of the negative control group,the flora diversity and richness of the model group had an upward trend,but there was no statistically significant difference.Compared with those of the model group,the flora diversity and relative abundance of Shegan Zhike Capsule groups had a downward trend,and were close to those of the negative control group,but there was no statistically significant difference.Firmicutes,Bacteroidetes and Proteobacteria were the dominant bacteria in rats.Compared with the model group

关 键 词：射干止咳胶囊 肠道菌群 耐甲氧西林金黄色葡萄球菌 16SrDNA 

分 类 号：R259.631[医药卫生—中西医结合]