肝素钠生产废水对小球藻生长和营养物去除的影响  

Effects of Heparin Sodium Production Wastewater on the Growth,Biochemical Compositions,and Nutrients Removal of Chlorella sorokiniana

作　　者：何雨晴 王红霞 胡强 韩丹翔[1] 王岚[1] HE Yuqing;WANG Hongxia;HU Qiang;HAN Danxiang;WANG Lan(Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;College of Advanced Agriculture Sciences,University of Chinese Academy of Sciences,Beijing 100049,China;Institute for Advanced Study,Shenzhen University,Shenzhen 518000,China)

机构地区：[1]中国科学院水生生物研究所,湖北武汉430072 [2]中国科学院大学现代农业科学学院,北京100049 [3]深圳大学高等研究院,广东深圳518000

出　　处：《食品与生物技术学报》2024年第7期133-142,共10页Journal of Food Science and Biotechnology

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项项目(2019YFD0900302);国家自然科学基金项目(32061133009)。

摘　　要：肝素钠生产废水含有丰富的营养物,是潜在的微藻培养基。为探究其培养微藻的可行性,利用不同体积分数(10%~40%)肝素钠生产废水培养3株索罗金小球藻Chlorellasorokiniana(编号GT1、XY3-3、CMBB276),筛选出高耐受性的藻株CMBB276。探究该藻株在不同体积分数肝素钠生产废水中生长时的营养物去除过程、生化组成以及热值等参数变化,以期筛选出最适废水体积分数。结果表明,随着废水体积分数增加,藻株CMBB276的最高细胞密度、总氮(TN)去除率、碳水化合物和脂肪酸质量分数、蛋白质产率逐渐降低,而总有机碳(TOC)与TN去除速率以及蛋白质质量分数逐渐增加,TOC去除率和热值基本保持不变。最终确定最适废水体积分数为20%,培养结束时藻株CMBB276的细胞密度为6.4×10^(7)个/mL,TOC和TN去除率分别为83.3%和63.0%,蛋白质质量分数及产率为52.5%和22.22 mg/(L·d),热值为20.37 MJ/kg。因此,利用适宜体积分数的肝素钠生产废水培养小球藻能高效净化肝素钠生产废水中的TOC和TN;同时,废水培养的藻株CMBB276具有高蛋白质和热值等特征,在资源化利用方面极具潜力。Heparin sodium production wastewater(HSW)is rich in nutrients,making it a potential culture medium for microalgae.To explore its feasibility for microalgal cultivation,three strains of Chlorella sorokiniana(GT1,XY3-3,and CMBB276)were cultured in HSW at varying volume fractions(10%~40%),and Chlorella sorokiniana CMBB276 with high tolerance to HSW was screened.To determine the optimal volume fraction of wastewater,the author investigated the removal processes of nutrients,along with the variations in biochemical composition and calorific value,during the growth of the strain CMBB276 in HSW at different volume fractions.Results demonstrated that as the wastewater volume fraction increased,the maximum cell density,total nitrogen(TN)removal efficiency,mass fractions of carbohydrates and fatty acids,and protein productivity of CMBB276 gradually decreased,while the total organic carbon(TOC)and TN removal rates and protein mass fraction gradually increased.The TOC removal efficiency and calorific value remained relatively unchanged.The optimal wastewater volume fraction was determined to be 20%.At the end of cultivation,the cell density of CMBB276 was 6.4×10^(7) cells/mL,with TOC and TN removal efficiencies of 83.3%and 63.0%,respectively.The mass fraction and productivity of protein was 52.5%and 22.22 mg/(L·d),respectively,with a calorific value of 20.37 MJ/kg.Therefore,cultivating C.sorokiniana in HSW at an appropriate volume fraction can efficiently remove TOC and TN from the wastewater,while the cultured CMBB276 strain,characterized by its high protein content and calorific value,holds significant potential for resource utilization.

关 键 词：肝素钠 生产废水 索罗金小球藻 蛋白质 净化 资源化利用 

分 类 号：X703[环境科学与工程—环境工程]