甜樱桃绿变植原体的分子检测及鉴定  

Molecular detection and identification of sweet cherry virescence phytoplasma

作　　者：陈金霄 董晓旭 朱天生[1] 高瑞 王洁[2] CHEN Jinxiao;DONG Xiaoxu;ZHU Tiansheng;GAO Rui;WANG Jie(College of Plant Science,Tarim University/Joint Laboratory of Integrated Control of Agricultural Pests in Southern Xinjiang Production and Construction Corps/National-Local Joint EngineeringLaboratory for High-yield and High-quality Cultivation and Deep Processing of South Xinjiang Regional SpecialFruits,Alar,Xinjiang 843300,China;Shandong Institute of Pomology,Tai’an,Shandong 271000,China)

机构地区：[1]塔里木大学植物科学学院/南疆农业有害生物综合治理兵团重点实验室/南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室,新疆阿拉尔843300 [2]山东省果树研究所,山东泰安271000

出　　处：《落叶果树》2024年第5期12-15,共4页Deciduous Fruits

基　　金：兵团特色果树生产工程实验室开放课题(FE202001);兵团财政科技计划资助(2019DA001,2020DA003)。

摘　　要：为明确甜樱桃绿变植原体的分类地位,通过对表现绿变症状的甜樱桃感病植株总DNA进行16S rRNA基因、rp基因和tuf基因的PCR扩增、克隆及序列分析。结果表明,甜樱桃绿变植原体16SrRNA基因片段大小为1248 bp,与枣疯植原体(AB052876)聚为一簇,且相似系数为1.00,应同属于16SrⅤ-B亚组。基于rp基因系统进化分析表明,甜樱桃绿变植原体与枣疯植原体JWB(AY197681)聚为一簇,属于rpV-C亚组。基于tuf基因系统进化分析显示,甜樱桃绿变植原体与枣疯植原体JWB-XFDO(GU968759)等聚为一簇,属于16SrⅤ组。明确了甜樱桃绿变植原体的分类,为泰安地区病害检测、诊断以及科学防控提供依据。To confirm phytoplasma infection,samples of sweet cherry(Prunus avium L.)plants showing virescence symptom were collected from an orchard in Tai’an.Total DNAs were extracted from symptomatic and asymptomatic plants for PCR amplification.A 16SrDNA fragment of 1248 bp in length were amplified from diseased cherry tissue,but not from healthy plant,using universal primers for phytoplasmal 16SrRNA gene.The similarity coefficient between the SCHV and the 16SrV-B subgroup of jujube witches’-broom phytoplasma(AB052876)is 1.00.Phylogenetic analysis based on rp genes revealed that SCHV belonged to rpV-C subgroup.Sequence analysis indicated the tuf gene of SCHV was clustered with jujube witches’-broom phytoplasma JWB-XFDO(GU968759),belonging to the 16SrⅤgroup.In conclusion,this study clarified the classification of sweet cherry virescence phytoplasma isolated from Tai’an,providing a foundation for local disease detection,diagnosis,and scientific control.

关 键 词：甜樱桃 植原体 16S rRNA基因 rp基因 tuf基因 

分 类 号：S662.5[农业科学—果树学]