柑橘叶疱斑病毒樱桃株系的全基因组克隆和初步鉴定  

Whole genome cloning and preliminary identification of CLBV strain in Prunus

作　　者：王刚 乔谦 朱东姿 刘庆忠[1] 王甲威[1] WANG Gang;QIAO Qian;ZHU Dongzi;LIU Qingzhong;WANG Jiawei(Shandong Institute of Pomology/Ministry of Agriculture Huanghuai area Pomology Science Observation Experimental Station,Tai'an,Shandong 27100,China)

机构地区：[1]山东省果树研究所/农业部黄淮地区果树科学观测实验站,山东泰安271000

出　　处：《落叶果树》2024年第5期16-19,共4页Deciduous Fruits

基　　金：山东省自然科学基金面上项目(ZR2021MC117);烟台市大樱桃育种联合攻关团队创建项目;山东省重点研发计划项目(2022TZXD006)。

摘　　要：本研究利用small RNA高通量测序在甜樱桃中发现了与乙型线性病毒科柑橘病毒属的柑橘叶疱斑病毒具有较高同源性一种病毒。对该病毒进行全基因组克隆,分析发现,该病毒在核苷酸水平上与柑橘叶疱斑病毒的同源性为80%;该病毒的外壳蛋白基因序列与柑橘叶疱斑病毒的外壳蛋白基因序列进行同源性比较,在核苷酸水平上,同源性为79%,在氨基酸水平上,同源性为88%。根据该科病毒新种的划分标准,认为该病毒为柑橘叶疱斑病的一个新株系,将其命名为柑橘叶疱斑病毒樱桃株系。In this study,small RNA high-throughput sequencing was used to identify a virus with high homology to the citrus leaf blister virus(CLBV)of the genus beta-linear virus in sweet cherry.The whole genome of the virus was cloned,and it was found that the virus had 80%homology with CLBV at nucleotide level;the sequence homology of the coat protein gene of this virus was compared with that of CLBV.The homology was 79%at the nucleotide level and 88%at the amino acid level.According to the classification criteria,this virus was considered a new strain of CLBV,and named Citrus Leaf Blotch virus Strain Prunus.

关 键 词：柑橘叶疱斑病毒CLBV small RNA高通量测序 同源性分析 

分 类 号：S662.5[农业科学—果树学]