单纯前交叉韧带离断法建立兔膝骨关节炎模型  

Establishment of rabbit knee osteoarthritis model by simple anterior cruciate ligament disruption

作　　者：李顺利 赵永红 王新军 沈明球 LI Shun-li;ZHAO Yong-hong;WANG Xing-jun;SHEN Ming-qiu(Graduate School of Xinjiang Medical University,Urumqi 830001,Xinjiang,China;Xinjiang Aged Disease Hospital,Urumqi830001,Xinjiang,China;The Fourth Clinical College of Xinjiang Medical University,Urumqi830001,Xinjiang,China)

机构地区：[1]新疆医科大学研究生学院,新疆乌鲁木齐830001 [2]新疆老年病医院,新疆乌鲁木齐830001 [3]新疆医科大学第四临床医学院,新疆乌鲁木齐830001

出　　处：《中国骨伤》2024年第10期1003-1009,共7页China Journal of Orthopaedics and Traumatology

基　　金：新疆维吾尔自治区自然科学基金面上项目(编号:2020D01C142)。

摘　　要：目的:采用单纯前交叉韧带离断法建立兔膝骨关节炎(knee osteoarthritis,KOA)模型。方法:选取40只大白兔,随机分为空白组6只和造模组34只,造模组在局部麻醉下行右膝关节前交叉韧带离断术。在造模后4、8、12周时,宏观及微观观察大白兔关节软骨、髌下脂肪垫与滑膜变化,并进行大体观评分及组织病理学评分。结果:造模4、8、12周时,造模组关节软骨均有不同程度的退行性改变。在造模8、12周时,髌下脂肪垫及滑膜分别呈现中期、晚期KOA典型病理表现。空白组在相同时间段内关节软骨、髌下脂肪垫及滑膜无变化。造模组与空白组大体观评分在4周[(1.300±0.674)分vs 0.000分]、8周[(2.400±0.516)分vs 0.000分]、12周[(3.583±0.668)分vs 0.000分]差异有统计学有意义(P<0.05),造模组与空白组在4周[(2.900±0.567)分vs 0.000分]、8周[(7.500±1.178)分vs(0.500±0.707)分]、12周[(11.833±1.337)分vs(1.500±0.707)分]相关组织病理学评分差异有统计学有意义(P<0.05)。结论:单纯离断前交叉韧带建立兔KOA模型简单可行,稳定性好,成功率高,创伤小,造模周期短,动物生理结构改变较少,对不同分期、分级的KOA均有较好的造模效果,术后感染和创伤可控,能够满足实验需求。Objective The rabbit knee osteoarthritis(KOA)model was established by simple anterior cruciate ligament disruption.Methods The selected 40 adult white rabbits were randomly divided into two groups,blank group with 6 rabbits and model group with 34 rabbits.The rabbits in model group were given the disruption of anterior cruciate ligament of right knee joint.In the fourth,eighth and the twelfth weeks of modeling,different degrees of degenerative changes have been observed on the rabbits articular cartilages from both macroscopic and microscopic aspects.Results At 4,8 and 12 weeks after modeling,the articular cartilage of the rabbits in the model group showed degenerative changes in different degrees.At 8 and 12 weeks,the typical pathological manifestations of KOA in the middle and late stages were found in the infrapatellar fat pad and synovium,respectively.There were no changes in articular cartilage,infrapatellar fat pad and synovial membrane in the blank group at the same time.There were significant differences in the gross score between the model group and the blank group at 4 weeks[(1.300±0.674)vs 0.000],8 weeks[(2.400±0.516)vs 0.000]and 12 weeks[(3.583±0.668)vs 0.000](P<0.05),the histopathological scores were significantly different,at 4 weeks[(2.900±0.567)vs 0.000],8 weeks[(7.500±1.178)vs(0.500±0.707)],12 weeks[(11.833±1.337)vs(1.500±0.707)],(P<0.05).Conclusion The establishment of rabbit knee arthritis model by simply cutting the anterior cruciate ligament is simple and feasible,with good stability,high success rate,small trauma,short modeling cycle and less changes in animal physiological structure.The rabbit knee arthritis model established by simply cutting the anterior cruciate ligament has good modeling effect on different stages and grades of knee osteoarthritis,and the postoperative infection and trauma can be controlled,which can meet the test requirements.

关 键 词：膝骨关节炎 动物模型 前交叉韧带 

分 类 号：R274.9[医药卫生—中西医结合]