Unraveling the mechanism of action of Shangxia Liangji formula for treating insomnia:a metabolomics and network pharmacology approach  

作　　者：Xia-Jie Quan Hao Liang Yong-Hong Tang Li Jiang Xiong-Ying Ji Feng-Ying Zhang Ping Zhang Bo Ouyang 

机构地区：[1]Department of Information,The Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China [2]Department of Neurology,The Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China [3]Department of Gastroscopy,The Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China [4]Department of Traditional Chinese Medicine,The Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China

出　　处：《Traditional Medicine Research》2025年第2期16-29,共14页TMR传统医学研究

基　　金：Science Foundation of Hunan Province(2021JJ40510);General Guidance Project of Hunan Health Commission(202203074169);Clinical Medical Technology Innovation Guidance Project of Hunan Province(2021SK51901);and Key Guiding Projects of Hunan Health Commission(20201918)for supporting this study.

摘　　要：Background:Insomnia is a prevalent clinical condition and Shangxia Liangji formula(SXLJF)is a well-established method of treatment.Nevertheless,the specific mechanism of action of SXLJF remains unclear.Methods:The mouse model of insomnia was established by intraperitoneal injection of para-chlorophenylalanine.Forty-two mice were randomly divided into a negative control group,model group,SXLJF group(18.72 g/kg/day),and positive control group(diazepam,2 mg/kg)and treated with the corresponding drugs for 7 consecutive days.The open field test and pentobarbital-induced sleeping test were conducted.LC-MS-based untargeted metabolomics and network pharmacology were applied to explore the potential targets of SXLJF for treating insomnia.Finally,key targets were validated using RT-qPCR.Results:Behavioral tests demonstrated that SXLJF reduced the total distance,average velocity,central distance,and sleep latency,and prolonged sleep duration.Metabolomics and network pharmacology revealed potential targets,signaling pathways,metabolic pathways,and metabolites associated with the anti-insomnia effects of SXLJF.Specifically,tyrosine hydroxylase(TH)and tyrosine metabolism emerged as crucial metabolic pathways and targets,respectively.RT-qPCR results supported the role of TH in the mechanism of SXLJF in treating insomnia.Conclusion:In conclusion,TH and tyrosine metabolism may represent significant targets and pathways for SXLJF in treating insomnia.

关 键 词：Shangxia Liangji formula INSOMNIA metabolomics network pharmacology tyrosine hydroxylase tyrosine metabolism 

分 类 号：R285[医药卫生—中药学]