SDC1基因单核苷酸多态性与汉族孕产妇伴有严重特征的子痫前期易感性相关性研究  

The correlation between single nucleotide polymorphism of SDC1 gene and susceptibility to preeclampsia with severe features in Han pregnant women

作　　者：谭家余 黄湘[2] 钟苑芳 蔡凤娥[4] 甘玉杰[4] Tan Jiayu;Huang Xiang;Zhong Yuanfang(Department of Emergency,Foshan Women and Children Hospital,Foshan 528200;Prenatal Diagnosis Center,Foshan Women and Children Hospital,Foshan 528200;Intensive Care Unit,Boai Hospital of Zhongshan City Affiliated Hospital to Southern Medical University,Zhongshan 528403)

机构地区：[1]佛山市妇幼保健院急诊科,佛山528200 [2]佛山市妇幼保健院产前诊断中心,佛山528200 [3]南方医科大学附属中山市博爱医院ICU,中山528403 [4]南方医科大学附属中山市博爱医院产科,中山528403

出　　处：《现代妇产科进展》2024年第11期830-833,837,共5页Progress in Obstetrics and Gynecology

基　　金：广东省中山市社会公益与基础研究专项(医疗卫生重点项目)(No:2020B3011)。

摘　　要：目的:探讨SDC1基因家族单核苷酸多态性(SNPs)与汉族孕妇伴有严重特征的子痫前期(PE-sf)易感性的相关性。方法:选取2020年5月至2022年4月中山市博爱医院收治的PE-sf孕产妇201例和健康孕产妇171例,利用飞行时间质谱检测系统对SDC1基因的4个SNPs位点(rs1131351、rs11899121、rs2230924和rs11544860)进行基因分型,探讨SDC1的SNPs对其编码产物血清syndecan-1浓度的影响。结果:与对照组相比,PE-sf组的血清syndecan-1水平明显降低,差异有统计学意义(P=0.006)。基因分型成功率为100%,其中rs11544860全部为GG基因型,未见AA、GA基因型,不符合Hardy-Weinberg遗传平衡定律。与对照组相比,PE-sf组rs1131351的等位基因分布差异有统计学意义(χ^(2)=5.342,P=0.021),rs2230924和rs11899121均无差异(χ^(2)=0.025,P=0.873;χ^(2)=0.053,P=0.818)。与对照组相比,PE-sf组rs1131351和rs2230924的基因型分布差异均有统计学意义(χ^(2)=14.581,P=0.001;χ^(2)=6.788,P=0.034),rs11899121则无差异(χ^(2)=0.155,P=0.926)。进行Bonferroni校正后rs1131351的基因型分布差异有统计学意义(P<0.05),rs2230924则无差异(P>0.05)。与CC基因型相比,rs1131351的CG和GG基因型均显著增加PE-sf发生的危险性,OR分别为2.731(1.616~4.617)和2.118(1.171~3.833)。经Spearman相关性检验,发现SDC1基因的rs1131351位点SNPs与血清syndecan-1水平具有相关性(r=-0.352,P=0.022)。与CC、GG基因型组相比,PE-sf患者CG基因型组的血清syndecan-1水平明显降低(均P<0.05)。结论:SDC1基因的SNPs可能与PE-sf易感性有关,CG基因型对血清syndecan-1浓度有一定影响。Objective:To investigate the correlation between single nucleotide polymorphism of SDC1 gene and susceptibility to preeclampsia with severe features(PE-sf)in Han pregnant women.Methods:From May 2024 to April 2022,201 pregnant women diagnosed with PE-sf and 171 healthy pregnant women were selected as the study subjects.The four SNPs sites(rs1131351,rs11899121,rs2230924 and rs11544860)of the SDC1 gene were genotyped using flight time mass spectrometry detection system,and the effect of SDC1 SNPs on serum syndecan-1 concentration was explored.Results:Compared with the control group,the serum syndecan-1 level in the PE-sf group was significantly decreased,with a significant difference(P=0.006).The success rate of genotyping was 100%,with rs11544860 being the GG genotype and no AA or GA genotypes observed,which does not comply with Hardy Weinberg's law of genetic balance.Compared with the control group,the allelic distribution of rs1131351 in the PE-sf group showed differences(χ^(2)=5.342,P=0.021),there was no difference in rs2230924 and rs11899121(χ^(2)=0.025,P=0.873;χ^(2)=0.053,P=0.818).Compared with the control group,the genotype distribution of rs1131351 and rs2230924 in the PE-sf group showed significant differences(χ^(2)=14.581,P=0.001;χ^(2)=6.788,P=0.034),there was no difference in rs11899121(χ^(2)=0.155,P=0.926).After Bonferroni correction,there was still a significant difference in the genotype distribution of rs1131351(P<0.05),while rs2230924 did not(P>0.05).Compared with the CC genotype,the CG and GG genotypes of rs1131351 significantly increase the risk of PE-sf,with OR values of 2.731(1.616~4.617)and 2.118(1.171~3.833),respectively.After Spearman correlation test,the rs1131351 SNPs of the SDC1 gene were correlated with the serum syndecan-1 level(r=-0.352,P=0.022).Compared with the CC and GG genotype groups,the serum syndecan-1 levels in the CG genotype group of PE-sf patients were significantly reduced(all P<0.05).Conclusion:The single nucleotide polymorphism of SDC1 gene may be related to PE-sf sus

关 键 词：子痫前期 SDC1 SYNDECAN-1 基因多态性 飞行时间质谱检测系统 

分 类 号：R714.4[医药卫生—妇产科学]