血清HMGB1、IFN-γ水平与再生障碍性贫血患儿细胞免疫障碍的相关性分析  

Correlation analysis of serum HMGB1 and IFN-γlevels with cellular immunity disorder in children with aplastic anemia

作　　者：郭玲慧 周会杰[1] Guo Ling-hui;Zhou Hui-jie(Department of Clinical Laboratory,Anyang District Hospital,Anyang 456500,Henan,China)

机构地区：[1]濮阳市安阳地区医院检验科,河南安阳456500

出　　处：《四川生理科学杂志》2024年第11期2502-2505,共4页

摘　　要：目的:探讨血清高迁移率族蛋B1(high mobility group box-1 protein,HMGB1)、干扰素-γ(Interferonγ,IFN-γ)水平与再生障碍性贫血患儿细胞免疫障碍的相关性。方法:回顾性选取我院2022年6月至2023年6月的再生障碍性贫血患儿115例作为研究组,其中重型再生障碍性贫血患儿48例,轻型再生障碍性贫血患儿67例。按照患儿接受治疗后的预后情况分为发生组和未发生组,其中发生组45例,未发生组70例;另选取在我院进行体检的100例健康儿童作为参照组。检测并对比各组血清HMGB1、IFN-γ水平,并对比轻型、重型再生障碍性贫血患儿血清HMGB1、IFN-γ、血小板计数(Platelet count,PLT)、巨核细胞计数(Megakaryocyte,MK)水平,分析再生障碍性贫血患儿血清HMGB1、IFN-γ水平和PLT、MK之间的关系,对比预后不同状况两组的血清HMGB1、IFN-γ水平,采用ROC曲线分析血清HMGB1、IFN-γ预测再生障碍性贫血患儿细胞免疫障碍的临床价值。结果:研究组血清HMGB1、IFN-γ水平均比参照组高(P<0.05);重型再生障碍性贫血组血清HMGB1、IFN-γ水平均相较于轻型组明显上升,而重型再生障碍性贫血组PLT、MK均相较于轻型组下降(P<0.05);再生障碍性贫血患儿的血清HMGB1和PLT、MK均呈负相关(P<0.05);血清IFN-γ与PLT、MK均呈负相关(P<0.05);发生组血清HMGB1、IFN-γ水平相较于未发生组升高(P<0.05);血清HMGB1、IFN-γ联合预测再生障碍性贫血患儿细胞免疫障碍AUC值为0.911,比单独采用HMGB1、IFN-γ预测的AUC值高。结论:再生障碍贫血患儿血清HMGB1、IFN-γ水平异常上升,且上述指标和细胞免疫障碍之间有着密不可分的关系,可将其作为再生障碍性贫血患儿细胞免疫障碍评估的主要指标。Objective:To investigate the correlation between serum high mobility group box-1 protein(HMGB1)and Interferonγ(IFN-γ)levels and cellular immunity disorder in children with aplastic anemia.Methods:115 children with aplastic anemia in our hospital from June 2022 to June 2023 were retrospectively selected as the study group,including 48 children with severe aplastic anemia and 67 children with mild aplastic anemia.According to the prognosis after treatment,the children were divided into occurrence group and non-occurrence group,including 45 cases in occurrence group and 70 cases in non-occurrence group.100 healthy children who underwent physical examination in our hospital were selected as the control group.Serum HMGB1 and IFN-γlevels of all subjects were detected and compared.And serum HMGB1,IFN-γ,platelet count(PLT)and megakaryocyte(MK)levels of children with mild and severe aplastic anemia were compared.The relationship between serum HMGB1 and IFN-γlevels and PLT and MK in children with aplastic anemia was analyzed.Serum HMGB1 and IFN-γlevels in the two groups with different prognosis were compared,and the clinical value of serum HMGB1 and IFN-γin predicting cellular immune disorders in children with aplastic anemia was analyzed by ROC curve.Results:The serum levels of HMGB1 and IFN-γin the study group were higher than those in the control group(P<0.05).Serum levels of HMGB1 and IFN-γin severe group were significantly increased compared with those in mild group,while PLT and MK were decreased in severe group compared with those mild group(P<0.05).Serum HMGB1 was negatively correlated with PLT and MK in children with aplastic anemia(P<0.05).Serum IFN-γwas negatively correlated with PLT and MK(P<0.05).Serum levels of HMGB1 and IFN-γin occurrence group were higher than those in non-occurrence group(P<0.05).The AUC value of serum HMGB1 and IFN-γcombined predicted cellular immune disorder in children with aplastic anemia was 0.911.The AUC value was higher than that predicted by HMGB1 and IFN-γalone.Conclu

关 键 词：高迁移率族蛋B1 干扰素-Γ 再生障碍性贫血 细胞免疫障碍 相关性 

分 类 号：R556[医药卫生—血液循环系统疾病]