猕猴桃果粉的降脂作用研究  

Study on the lipid-lowering effect of kiwi fruit powder

作　　者：蔡露阳[1] 王科堂 陈雪峰[1,2] 陈梦音 赵燕妮 付欢 李永宁 CAI Luyang;WANG Ketang;CHEN Xuefeng;CHEN Mengyin;ZHAO Yanni;FU Huan;LI Yongning(School of Food Science and Engineering,Shaanxi University of Science and Technology,Xi’an 710021,China;Shaanxi Agricultural Product Processing Technology Research Institute,Xi’an 710021,China)

机构地区：[1]陕西科技大学食品科学与工程学院,陕西西安710021 [2]陕西农产品加工技术研究院,陕西西安710021

出　　处：《食品与发酵工业》2024年第21期287-293,共7页Food and Fermentation Industries

基　　金：陕西省科技厅重点研发一般项目(2022NY-014);陕西省农业厅科技创新驱动项目(NYKJ-2022-XA-09,NYKJ-2021-XA-003)。

摘　　要：高血脂症严重威胁人类健康,亟待开发安全无副作用的治疗高血脂辅助健康食品,该文从体内角度探究猕猴桃果粉降脂作用,旨在为猕猴桃果粉辅助治疗高血脂症,实现其高附加值利用提供理论依据。将小鼠随机分为5组,高脂饲料组(MD),低、高剂量猕猴桃果粉灌胃组(LFP、HFP),辛伐他汀阳性对照组(BK)及正常饲料的空白组(PC),进行为期13周的饲养后,对比不同组小鼠体重、脏器指数,测定葡萄糖耐受量、小鼠血清生化指标、肝脏生化指标、体内瘦素和炎症因子含量;通过苏木精-伊红染色以及油红O染色显微镜观察小鼠肝脏组织切片,对猕猴桃果粉降脂作用进行深入研究。结果表明,MD组各项指标均出现异常,且和其余各组相比存在显著性差异(P<0.05);与MD组相比,灌胃猕猴桃果粉后,小鼠各项指标均得到改善,且量效关系明显,与PC组相比,HFP组小鼠肝脏指数、总胆固醇、低密度脂蛋白胆固醇、丙氨酸转氨酶、天冬氨酸转氨酶、瘦素指标无显著性差异(P>0.05),炎症因子含量降低;HFP组小鼠肝脏细胞结构清晰,无明显变形,无明显的肝窦扩张,脂滴基本消失不见;说明猕猴桃果粉具调节脂质代谢、减轻肝损伤、缓解高血脂症的作用。Obesity seriously affects human health,it is urgent to develop a safe and healthy food to treat hyperlipidemia adjuvant without side effects.In this paper,the lipid lowering effect and mechanism of kiwi fruit powder were studied in vivo.The aim is to provide theoretical basis for kiwi fruit powder to assist the treatment of hyperlipidemia and realize its high value-added utilization.The mice were randomly divided into 5 groups:high-fat diet group(MD),low-dose and high-dose kiwi fruit powder gavage group(LFP and HFP),positive control group(simvastatin BK)and normal diet blank group(PC).After feeding for 13 weeks,the body weight and organ index of different groups of mice were compared.Glucose tolerance,serum biochemical indexes,liver biochemical indexes,and the contents of leptin and inflammatory factors in vivo were measured.The liver sections of mice were observed by hematoxylin-eosin staining and oil red O staining.The effect of kiwi fruit powder on lipid lowering and its mechanism were studied.The results showed that all indexes of high-fat diet group were abnormal,and there were significant differences compared with other groups(P<0.05).Compared with MD group,all indexes of mice were improved after administration of kiwi fruit powder,and the dose-effect relationship was obvious.There were no significant differences in liver index,total cholesterol,low density lipoprotein cholesterol,alanine aminotransferase,aspartate transaminase and leptin compared with PC group(P>0.05).The liver cell structure of mice in HFP group was clear.There was no obvious deformation,no obvious expansion of hepatic sinuses,and the lipid droplets basically disappeared.The results indicated that kiwi fruit powder could reduce weight and lipid,regulate lipid metabolism and reduce liver injury.

关 键 词：猕猴桃果粉 高血脂症 降脂 作用机制 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]