支气管肺发育不良患儿血清miR-146b及炎性因子表达的临床意义  

Clinical significance of expression of serum miR-146b and inflammatory factors for children with bronchopulmonary dysplasia

作　　者：刘瑾 龙运峰 罗勇 刘辉[1] LIU Jin;LONG Yunfeng;LUO Yong;LIU Hui(Department of Neonatology,The First Affiliated Hospital of Shaoyang University,Shaoyang 422000,China)

机构地区：[1]邵阳学院附属第一医院新生儿科,湖南邵阳422000

出　　处：《邵阳学院学报（自然科学版）》2024年第5期8-14,共7页Journal of Shaoyang University：Natural Science Edition

基　　金：湖南省科技厅科卫联合项目(2022JJ70040)。

摘　　要：目的探讨支气管肺发育不良(bronchopulmonary dysplasia,BPD)患儿中miR-146b和炎性因子表达水平的临床价值和意义。方法收集邵阳学院附属第一医院新生儿科2022年期间住院早产儿62例,根据支气管肺发育不良诊断标准,将纳入的早产儿分为BPD组和非BPD组。收集其脐带血以及出生后的不同时间点的血样本。检测血样本中miR-146b、白介素(interleukin-1β,IL-1β)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白介素-6(interleukin-6,IL-6)的表达水平。结果非BPD组生后各时间点血清miR-146b水平均高于BPD组,差异均有统计学意义(均P<0.05)。BPD组患儿在生后3 d血清miR-146b水平降至最低,随后逐渐上升,在生后14~28 d维持在低表达水平。非BPD组患儿在生后3~7 d血清miR-146b表达水平逐渐下降至最低,随后表达水平相对稳定,但各个时间点的表达水平均高于BPD组。非BPD组不同时间点的炎性细胞因子IL-1β、TNF-α、IL-6的表达水平均低于BPD组。BPD组TNF-α在生后3 d达峰值,随后下降,但仍然保持高水平表达;IL-1β在脐血中表达水平极高,随着日龄增长,逐渐下降;IL-6生后3~14 d的表达稳定,28 d明显下降,但是仍然高于非BPD组(均P<0.05)。结论在BPD的进程中,低表达水平的miR-146b可能促进BPD的炎症发展;而高表达水平的miR-146b可能阻止这一进程,为BPD的治疗提供了潜在靶点。Objective To explore the clinical value and significance of miR-146b and inflammatory factor expression levels in children with bronchopulmonary dysplasia(BPD).Methods A total of 62 premature infants admitted to the Department of Neonatology of the First Affiliated Hospital of Shaoyang University in 2022 were collected.According to the diagnostic criteria for BPD,they were divided into a BPD group and a non BPD group.Umbilical cord blood samples and blood samples at different time points after birth were collected to detect the expression levels of miR-146b interleukin-1β(IL-1β),tumor necrosis factorα(TNF-α),and interleukin-6(IL-6)in blood samples.Results The expression levels of serum miR-146b in the non BPD group were higher than that in the BPD group at all time points after birth,and the differences were statistically significant(all P<0.05).On the third day after birth,the expression levels of serum miR-146b in the BPD group decreased to the lowest level,maintaining low from the 14th to the 28th day after birth,while that in the non BPD group gradually fell to the bottom on the 3rd to the 7th day after birth,and remained relatively stable,higher at all time points was than that in the BPD group.Moreover,the expression levels of inflammatory cytokine IL-1β,TNF-α and IL-6 in the non BPD group were lower than in the BPD group.In the BPD group,the expression level of TNF-α reached the peak on the third day after birth,then decreased,but remained at a high level,the expression level of IL-1βin cord blood was extremely high and gradually decreased over time,and the expression level of IL-6 remained stable from the 3rd to the 14th day after birth,with a significant decrease on the 28th day,but was still higher than that in the non BPD group(all P<0.05).Conclusion As BPD develops,low expression levels of miR-146b may promote the inflammatory development of BPD,but high expression levels of miR-146b may hinder this process and provide potential targets for the treatment of BPD.

关 键 词：早产儿 miR-146b 炎性因子 

分 类 号：R722.1[医药卫生—儿科]