基于网络药理学的L-茶氨酸调节细胞因子分泌机制研究  

Research on the mechanism of L-theanine regulating lymphocyte cytokine secretion based on network pharmacology

作　　者：李成舰 欧阳香 黄春花 赵金诚 于旭东 LI Chengjian;OUYANG Xiang;HUANG Chunhua;ZHAO Jincheng;YU Xudong(School of Pharmacy,Shaoyang University,Shaoyang 422000,China;Hunan Engineering Research Center of Development and Utilization of Traditional Chinese Medicine in Southwest Hunan,Shaoyang 422000,China)

机构地区：[1]邵阳学院药学院,湖南邵阳422000 [2]湘西南中药开发利用湖南省工程研究中心,湖南邵阳422000

出　　处：《邵阳学院学报（自然科学版）》2024年第5期62-67,共6页Journal of Shaoyang University：Natural Science Edition

基　　金：湖南省自然科学基金(2022JJ50026)。

摘　　要：目的利用网络药理学方法预测L-茶氨酸调节细胞因子分泌的可能机制。方法Swiss Target Prediction和TargetNet数据库预测L-茶氨酸的作用靶点,GeneCards数据库、TTD数据库和OMIM数据库预测免疫细胞因子分泌相关靶点,在线工具Venny 2.1求两者的交集靶点,对交集靶点进行PPI分析以及GO富集分析和KEGG富集分析。结果网络药理学结果显示,L-茶氨酸和免疫细胞因子分泌之间的共同靶点81个。其中,IL-1B、TLR4、NFκB1、MMP9、EGFR、JUN等核心靶点可能参与了L-茶氨酸的作用。KEGG富集分析进一步表明,L-茶氨酸的作用可能与雌激素信号通路、松弛素信号通路、cAMP信号通路和cGMP-PKG信号通路有关。结论L-茶氨酸调节细胞因子分泌的机制可能与L-茶氨酸作用于IL-1B、TLR4、NFκB1、MMP9、EGFR、JUN等靶点进而调节雌激素信号通路、松弛素信号通路、cAMP信号通路和cGMP-PKG信号通路有关。Objective The potential mechanism of L-theanine in regulating cytokine secretion was predicted using network pharmacology methods.Methods The Swiss Target Prediction and TargetNet databases were utilized to predict the targets of L-theanine,while the GeneCards,TTD,and OMIM databases were used to predict targets related to immune cytokine secretion.Finally,the online tool Venny 2.1 was employed to identify their intersection,which was then subjected to PPI analysis,GO enrichment analysis,and KEGG enrichment analysis.Results The network pharmacology results revealed 81 common targets of L-theanine and immune cytokine secretion.Among these,core targets such as IL-1B,TLR4,NFκB1,MMP9,EGFR,and JUN may be involved in the action of L-theanine.KEGG enrichment analysis further suggested that the effects of L-theanine may be associated with the estrogen signaling pathway,relaxin signaling pathway,cAMP signaling pathway,and cGMP-PKG signaling pathway.Conclusion The mechanism by which L-theanine regulates cytokine secretion may be related to its action on targets such as IL-1B,TLR4,NFκB1,MMP9,EGFR,and JUN,thereby modulating the estrogen,relaxin,cAMP,and cGMP-PKG signaling pathways.

关 键 词：L-茶氨酸 细胞因子分泌 网络药理学 免疫调节 信号通路 

分 类 号：R392.1[医药卫生—免疫学]