女性原发性干燥综合征患者外周血中雌激素及其受体水平的相关研究  

Study on estrogen and its receptors in peripheral blood of female patients with primary Sj gren's syndrome

作　　者：谢青青 李梦兰 肖东琴 帅世全 XIE Qingqing;LI Menglan;XIAO Dongqin;SHUAI Shiquan(Inflammation and Immunology Key Laboratory of Nanchong,Beijing Anzhen Nanchong Hospital,Capital Medical University,Nanchong Central Hospital,Nanchong 637000,Sichuan,China;Department of Immunology and Rheumatology,Beijing Anzhen Nanchong Hospital,Capital Medical University,Nanchong Central Hospital,Nanchong 637000,Sichuan,China;Institute of Tissue Engineering and Stem Cell Research,Beijing Anzhen Nanchong Hospital,Capital Medical University,Nanchong Central Hospital,Nanchong 637000,Sichuan,China)

机构地区：[1]首都医科大学附属北京安贞医院南充医院·南充市中心医院炎症与免疫南充市重点实验室,四川南充637000 [2]首都医科大学附属北京安贞医院南充医院·南充市中心医院风湿免疫科,四川南充637000 [3]首都医科大学附属北京安贞医院南充医院·南充市中心医院组织工程与干细胞研究所,四川南充637000

出　　处：《西部医学》2024年第11期1615-1619,共5页Medical Journal of West China

基　　金：国家自然科学基金资助项目(青年科学基金)(82002289);南充市2022年第二批市级科技计划项目(22JCYJPT0013)。

摘　　要：目的探讨雌激素(E)及其受体(ER)在绝经后原发性干燥综合征(pSS)患者外周静脉血中的变化及其可能的作用。方法收集2019年11月—2021年10月南充市中心医院风湿免疫科门诊及住院部收治的60例绝经后pSS患者(pSS组)和60例绝经后健康体检者(HC组)的临床资料和实验室检测指标,以及空腹外周静脉血,运用酶联免疫吸附(ELISA)检测两组血浆中17-雌二醇(E_(2))水平,实时荧光定量多聚酶链反应(RT-qPCR)检测两组外周血单个核细胞(PBMCs)中雌激素受体α(ERα)、雌激素受体β(ERβ)、G蛋白耦联受体30(GPR30)mRNA表达水平,免疫印迹试验(Western blot)检测两组PBMCs中ERα、ERβ、GPR30蛋白表达水平,采用SPSS 17.0统计软件进行分析,计量资料组间比较采用秩和检验和t检验,变量间相关关系采用Spearman相关分析。结果pSS组血浆中E_(2)均值水平低于HC组(P<0.01);pSS组ERα、ERβmRNA均显著低于HC组,GPR30 mRNA显著高于HC组(均P<0.01);Spearman相关性分析发现pSS组PBMCs中GPR30 mRNA与球蛋白(GLB)呈正相关(r=0.411,P=0.001);pSS组ERα蛋白表达水平与HC组比较差异无统计学意义(P>0.05),ERβ蛋白表达水平显著低于对照组(P<0.01),GPR30蛋白表达水平显著高于HC组(P<0.05)。结论雌激素及其受体在pSS患者外周血中存在差异表达,提示其可能参与了pSS的发生发展。Objective To explore the alterations of estrogen and its receptors in peripheral venous blood of postmenopausal patients with primary Sj gren's syndrome(pSS)and their possible roles.Methods The clinical data and laboratory test indexes of 60 postmenopausal pSS patients and 60 postmenopausal healthy subjects were collected,and fasting peripheral venous blood was collected.The plasma E_(2) level of the two groups was detected by enzyme-linked immunosorbent assay.The mRNA expression levels of ERα,ERβand GPR30 in peripheral blood mononuclear cells of the two groups were detected by real-time fluorescent quantitative polymerase chain reaction.The protein expression levels of ERα,ERβand GPR30 in PBMCs of the two groups were detected by Western blot.SPSS17.0 statistical software was used to analyze the data.Rank sum test and t test were used to compare the measurement data between groups,and Spearman correlation analysis was used to analyze the correlation between variables.Results The mean level of plasma E_(2) in pSS group was lower than that in HC group(P<0.01).ERαand ERβmRNA in pSS group were significantly lower than those in HC group(P<0.01),while GPR30 mRNA in pSS group was significantly higher than that in HC group(P<0.01).Spearman correlation analysis found that GPR30 mRNA was positively correlated with GLB in PBMCs of pSS group(r=0.411,P=0.001).There was no significant difference in ERαprotein expression level between pSS group and control group(P>0.05),ERβprotein expression level was significantly lower than that of control group(P<0.01),but GPR30 protein expression level was significantly higher than that of control group(P<0.05).Conclusion The differential expression of estrogen and its receptors in peripheral blood of patients with primary Sjogren's syndrome suggests that estrogen and its receptors may be involved in the occurrence and development of pSS.

关 键 词：原发性干燥综合征 雌激素 雌激素受体Α 雌激素受体Β G蛋白偶联受体30 

分 类 号：R593.2[医药卫生—内科学]