Temperature regulates negative supercoils to modulate meiotic crossovers and chromosome organization  

作　　者：Yingjin Tan Taicong Tan Shuxian Zhang Bo Li Beiyi Chen Xu Zhou Ying Wang Xiao Yang Binyuan Zhai Qilai Huang Liangran Zhang Shunxin Wang 

机构地区：[1]Advanced Medical Research Institute,Shandong University,Jinan 250012,China [2]State Key Laboratory of Reproductive Medicine and Offspring Health,Center for Reproductive Medicine,Institute of Women,Children and Reproductive Health,Shandong University,Jinan 250012,China [3]Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology,School of Life Sciences,Shandong University,Qingdao,Shandong 266237,China [4]Center for Cell Structure and Function,Shandong Provincial Key Laboratory of Animal Resistance Biology,College of Life Sciences,Shandong Normal University,Jinan 250014,China [5]National Research Center for Assisted Reproductive Technology and Reproductive Genetics,Shandong University,Jinan 250012,China [6]Key Laboratory of Reproductive Endocrinology(Shandong University),Ministry of Education,Jinan 250012,China [7]Shandong Technology Innovation Center for Reproductive Health,Jinan 250012,China [8]Shandong Provincial Clinical Research Center for Reproductive Health,Jinan 250012,China [9]Shandong Key Laboratory of Reproductive Medicine,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250012,China [10]Research Unit of Gametogenesis and Health of ART-Offspring,Chinese Academy of Medical Sciences(No.2021RU001),Jinan 250012,China

出　　处：《Science China(Life Sciences)》2024年第11期2426-2443,共18页中国科学（生命科学英文版）

基　　金：funded by the National Natural Science Foundation of China(32225015,32070837,32370907,32070575,32270895);the National Key Research and Developmental Program of China(2022YFC2702602,2021YFC2700103);the Taishan Scholars Program of Shandong Province(tstp20231256).

摘　　要：Crossover recombination is a hallmark of meiosis that holds the paternal and maternal chromosomes(homologs)together for their faithful segregation,while promoting genetic diversity of the progeny.The pattern of crossover is mainly controlled by the architecture of the meiotic chromosomes.Environmental factors,especially temperature,also play an important role in modulating crossovers.However,it is unclear how temperature affects crossovers.Here,we examined the distribution of budding yeast axis components(Red1,Hop1,and Rec8)and the crossover-associated Zip3 foci in detail at different temperatures,and found that both increased and decreased temperatures result in shorter meiotic chromosome axes and more crossovers.Further investigations showed that temperature changes coordinately enhanced the hyperabundant accumulation of Hop1 and Red1 on chromosomes and the number of Zip3 foci.Most importantly,temperature-induced changes in the distribution of axis proteins and Zip3 foci depend on changes in DNA negative supercoils.These results suggest that yeast meiosis senses temperature changes by increasing the level of negative supercoils to increase crossovers and modulate chromosome organization.These findings provide a new perspective on understanding the effect and mechanism of temperature on meiotic recombination and chromosome organization,with important implications for evolution and breeding.

关 键 词：MEIOSIS negative supercoils TEMPERATURE CHROMOSOME CROSSOVER Pds5 Hop1 Red1 Rec8 

分 类 号：Q343[生物学—遗传学]