Mechanism of Tea Polyphenols in Alleviating Thermal Damage Based on Network Pharmacology  

作　　者：Zhou Huishuang Lin Shuqian Li Guiming Liu Yueyue Yang Shifa Zhao Zengcheng Huang Zhongli Lian Ruirui Wang Yunchao Wan Renzhong Yin Bin 

机构地区：[1]Veterinary Pharmacology and Toxicology,College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai'an 271018,China [2]Poultry Institute,Shandong Academy of Agricultural Sciences,Jinan 250023,China [3]Shandong Provincial Engineering Center for Animal Health Products,Jinan 250023,China [4]Blue Horizon Eco-agriculture,Dongying 257091,China

出　　处：《Animal Husbandry and Feed Science》2024年第1期1-8,共8页动物与饲料科学（英文）

基　　金：Supported by National Natural Science Foundation of China(32302919,32302918);Taishan Industrial Experts Program(tscx202306046);Key R&D Program Rural Revitalization Project of Shandong Province(2023TZXD083);Science and Technology Cooperation Project of Shandong and Chongqing(2022LYXZ030)。

摘　　要：[Objective]This paper was to investigate the action targets and pathways of tea polyphenols in alleviating heat stress-induced injury by using network pharmacological analysis and an H9C2 cell model.[Method]First,the corresponding targets of tea polyphenols were obtained from the PubChem database.Then,the core targets were screened based on topological parameters.The relevant metabolism pathways of tea polyphenols related to diseases were identified through GO functional annotation and KECG signaling pathway enrichment.Moreover,common targets for thermal injury and targets of tea polyphenols were obtained.Then,GO functional annotation was performed to explore the pathway of tea polyphenols in alleviating heat stress damage.H9C2 cells were cultured at 42℃ to construct the heat stress model,and the cells were treated with 10μg/mL tea polyphenols.The key genes were confirmed using RT-PCR technology.[Result]The study yielded 364 targets corresponding to tea polyphenols,including 68 core targets.These targets are related to various biological processes such as involve oxidative stress,cancer,lipopolysaccharide-mediated signaling pathways,antiviral responses,regulation of cellular response to heat,apoptosis,and cellular lipid metabolic metabolism.Tea polyphe nols alleviate thermal damage by targeting BCL2,HSP90AA1,HSPA1A,JUN,MAPK1,NFKB1,NFKBIA,NOS3,and TP53.Moreover,10 mg/L tea polyphenols were found to upregulate the transcription levels of Hsp70,HO-1,NQ-O1,Nrf2,and MAPKI,and the transcription levels of Bax/Bcl2,p38,and JNK were downregulated to alleviate the heat stress-induced injury.[Conclusion]Tea polyphenols may enhance the antioxidant ability of H9C2 cells and inhibit cell apoptosis,thereby reducing heat stress injury.

关 键 词：Tea polyphenols H9C2 Network pharmacology Thermal damage 

分 类 号：S823.5[农业科学—畜牧学]