哈茨木霉与枯草芽孢杆菌对海红果幼苗生长、光合特性和抗氧化酶活性的影响  

Effects of Trichoderma harzianum and Bacillus subtilis on the growth,photosynthetic characteristics,and antioxidant enzyme activity of Malus micromalus Makino seedlings

作　　者：淡振荣 王洁琼 刘子贤 张志华 赵洪 杨靖芸 华小梅 贺利兵 钟子君 DAN Zhenrong;WANG Jieqiong;LIU Zixian;ZHANG Zhihua;ZHAO Hong;YANG Jingyun;HUA Xiaomei;HE Libing;ZHONG Zijun(Yulin Vocational and Technical College,Yulin 719000,China;Jujinbang Agricultural Products Development Company of Fugu County,Fugu 719400,China;Agricultural Environmental Protection Monitoring Station of Jingbian County,Jingbian 718500,China;Agricultural and Animal Husbandry Science and Technology Demonstration Center of Jingbian County,Jingbian 718500,China;Development and Reform and Science and Technology Bureau of Fugu County,Fugu 719400,China;Agricultural Technology Promotion Center of Dingbian County,Dingbian 718600,China)

机构地区：[1]榆林职业技术学院,陕西榆林719000 [2]府谷县聚金邦农产品开发公司,陕西府谷719400 [3]靖边县农业环境保护监测站,陕西靖边718500 [4]靖边县农牧业科技示范中心,陕西靖边718500 [5]府谷县发展改革和科技局,陕西府谷719400 [6]定边县农业技术推广中心,陕西定边718600

出　　处：《北方农业学报》2024年第4期103-112,共10页Journal of Northern Agriculture

基　　金：陕西省榆林市科学技术局项目“海红果有机生产关键技术示范与研究”(2023-CXY-216);陕西省科学技术厅重点研发项目“陕西省府谷县海红果栽培及精深加工技术研究与示范”(2023-YBNY-067)。

摘　　要：【目的】探究哈茨木霉和枯草芽孢杆菌接种对海红果幼苗生长、光合特性和抗氧化酶活性的影响。【方法】以海红果幼苗作为试验对象进行盆栽试验,设置施用无菌水(CK)、接种哈茨木霉菌液(TH)、接种枯草芽孢杆菌菌液(BS)、哈茨木霉菌液与枯草芽孢杆菌菌液混合接种(TB)4个处理,分析接种后30 d和60 d对海红果幼苗生长指标,叶片SPAD值、气体交换参数以及抗氧化酶活性和丙二醛(MDA)含量的影响。【结果】TH、BS、TB处理均促进了海红果幼苗的生长、光合特性及抗氧化酶活性,TB处理效果最佳。与CK相比,接种30 d和60 d,TB处理幼苗株高、茎粗、叶片数、地上部干重、根干重、根长分别显著增加16.52%、7.43%、23.81%、13.71%、21.44%、31.06%和16.27%、16.54%、18.06%、9.36%、15.41%、24.18%(P<0.05);幼苗叶片SPAD值显著增加13.38%和10.85%(P<0.05),净光合速率、气孔导度、蒸腾速率、胞间CO_(2)浓度分别显著增加14.08%、14.08%、31.03%、9.84%和11.88%、27.13%、14.95%、10.92%(P<0.05),过氧化物酶、苯丙氨酸解氨酶、超氧化物歧化酶、过氧化氢酶、多酚氧化酶活性分别显著增加20.93%、30.10%、42.00%、21.52%、23.71%和21.21%、22.52%、35.39%、18.18%、18.11%(P<0.05);接种60 d,MDA含量显著下降9.54%(P<0.05)。【结论】哈茨木霉与枯草芽孢杆菌混合接种可以有效促进海红果幼苗生长、提高叶绿素含量和光合速率、增强抗氧化酶活性。【Objective】To investigate the effects of inoculation with Trichoderma harzianum and Bacillus subtilis on the growth,photosynthetic characteristics,and antioxidant enzyme activity of Malus micromalus Makino seedlings.【Methods】A pot experiment was conducted on Malus micromalus Makino seedlings as experimental subjects,with four treatments,application of sterile water(CK),inoculation of T.harzianum suspension(TH),inoculation of B.subtilis suspension(BS),and mixed inoculation of T.harzianum suspension and B.subtilis suspension(TB).The effects of growth indicators,leaf SPAD values,gas exchange parameters,antioxidant enzyme activity,and malondialdehyde(MDA)content of Malus micromalus Makino seedlings were analyzed at 30 and 60 days after inoculation.【Results】TH,BS and TB treatments all promoted the growth,photosynthesis,and antioxidant enzyme activity of Malus micromalus Makino seedlings,with TB treatment showing the best effect.Compared with CK,after 30 and 60 days of inoculation,TB treatment significantly increased seedling plant height,stem diameter,number of leaves,aboveground dry weight,root dry weight,and root length by 16.52%,7.43%,23.81%,13.71%,21.44%,31.06%and 16.27%,16.54%,18.06%,9.36%,15.41%,24.18%,respectively(P<0.05);the SPAD values of seedling leaves significantly increased by 13.38%and 10.85%(P<0.05);the net photosynthetic rate,stomatal conductance,transpiration rate,and intercellular CO_(2) concentration of seedling leaves significantly increased by 14.08%,14.08%,31.03%,9.84%and 11.88%,27.13%,14.95%,10.92%,respectively(P<0.05);the activities of peroxidase activity,phenylalanine ammonia-lyase activity,superoxide dismutase activity,catalase activity,and polyphenol oxidase activity significantly increased by 20.93%,30.10%,42.00%,21.52%,23.71%,and 21.21%,22.52%,35.39%,18.18%,and 18.11%,respectively(P<0.05);after 60 days of inoculation,the MDA content significantly decreased by 9.54%(P<0.05).【Conclusion】Mixed inoculation of Trichoderma harzianum and Bacillus subtilis can effectively promote t

关 键 词：哈茨木霉 枯草芽孢杆菌 海红果 幼苗生长 光合特性 抗氧化酶活性 

分 类 号：S661.4[农业科学—果树学]