茶素柠檬合剂抑制耳廓痤疮模型大鼠炎症反应的作用研究  

Mechanism of Tea-Lemon Mixture Inhibiting the Inflammatory Response of Auricular Acne Model Rats

作　　者：林宝珍[1] 陈子馨 罗文川 肖丽涓 陈志惠 南丽红[2] 严洁 LIN Baozhen;CHEN Zixin;LUO Wenchuan;XIAO Lijuan;CHEN Zhihui;NAN Lihong;YAN Jie(The People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350004,China;School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Minhou Vocational Technical School,Fuzhou,Fujian 350100,China)

机构地区：[1]福建中医药大学附属人民医院,福建福州350004 [2]福建中医药大学药学院,福建福州350122 [3]闽侯职业中专学校,福建福州350100

出　　处：《福建中医药》2024年第10期20-23,共4页Fujian Journal of Traditional Chinese Medicine

基　　金：福建省科技厅引导性项目(2021Y0034)。

摘　　要：目的研究茶素柠檬合剂对耳廓痤疮模型大鼠炎症反应的作用及机制。方法采用随机数字表法将32只雄性SD大鼠分为正常组8只和造模组24只。造模组左耳廓皮内注射痤疮丙酸杆菌液50μL,腹腔注射痤疮丙酸杆菌液1 mL建立大鼠耳廓痤疮模型,当痤疮模型判定分级≥1级,则判定为造模成功。造模成功后将造模组采用随机数字表法分为对照组、模型组和给药组,每组8只。在大鼠左耳廓正反两面,对照组外涂0.1%维A酸乳膏0.2 mL,给药组外涂茶素柠檬合剂0.5 mL,正常组和模型组外涂生理盐水,1次/d,连续14 d。末次给药2 h后抽取腹主动脉血和切取1/4左耳廓组织,采用HE染色法观察左耳廓痤疮组织病理形态学改变,采用游标卡尺测定干预前后左耳肿胀度;采用ELISA和Western blot分别测定血清和左耳廓痤疮组织中白细胞介素-1α(IL-1α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量和蛋白表达水平。结果与正常组比较,模型组左耳廓组织出现明显炎症细胞浸润,左耳肿胀度,血清和左耳廓痤疮组织中IL-1α、IL-1β、IL-6、TNF-α含量和蛋白表达水平均明显增加(P<0.05);与模型组比较,对照组和给药组左耳廓组织炎症细胞浸润均有不同程度的改善,左耳肿胀度,血清和左耳廓痤疮组织中IL-1α、IL-1β、IL-6、TNF-α含量和蛋白表达水平均明显减少(P<0.05)。对照组和给药组上述指标比较,差异无统计学意义(P>0.05)。结论茶素柠檬合剂可降低血清和左耳廓痤疮组织中炎症因子IL-1α、IL-1β、IL-6、TNF-α含量和蛋白表达水平,降低耳部肿胀,从而减轻痤疮炎症反应,发挥治疗痤疮的作用。Objective:To observe the effect of tea-lemon mixture on the inflammatory response of auricle acne model rats.Methods:Thirty-two male SD rats were divided into normal group(n=8)and model-making group(n=24)by random number table method.The rats of model-making group were injected with 50μL of propionibacterium acnes solution intradermally in the left auri‐cle and 1 mL of the same solution intraperitoneally to establish the auricular acne model rats.When the acne model was graded≥1,the model was considered successful.After that,the rats of model-making group were divided into model group,control group and drug administration group by random number table method,with 8 rats in each group.In the control group,0.2 mL of 0.1%retinoic acid cream was applied externally to the front and back of the left auricle of the rats,while 0.5 mL of teicoplanin lemon compound was applied to the rats in the drug administration group,and saline was applied to rats of the normal and model groups,once a day for 14 days.Two hours after the last administration,blood was drawn from the abdominal aorta and 1/4 of the left auricle tissue was cut off.HE staining was used to observe the pathological morphological changes of the left auricle acne tissue.Vernier caliper was used to measure the swelling of the left ear before and after intervention.ELISA and Western blot were used to determine the serum content and relative protein expressions of interleukin-1α(IL-1α),interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in serum and left auricle acne tissue,respectively.Results:Compared with the normal group,the model group showed obvious inflammatory cell infiltration in the left auricle tissue,and the left ear was swelling,the serum IL-1α,IL-1β,IL-6,TNF-αcontent and protein relative expression in the left auricle tissue significantly increased(P<0.05);compared with the model group,the control and drug administation groups showed different degrees of improvement in the inflammatory cell infiltration in the left

关 键 词：痤疮 茶素柠檬合剂 炎症因子 耳部肿胀度 

分 类 号：R285.5[医药卫生—中药学]