血清补体C3糖基化修饰与糖尿病视网膜病变关系的研究  

Study on Association of Serum Complement C3 Glycosylation with Diabetic Retinopathy

作　　者：袁钟浩 田洪涛 赖治臻 于伟泓[2] 周江[3] 李智立[1] YUAN Zhong-hao;TIAN Hong-tao;LAI Zhi-zhen;YU Wei-hong;ZHOU Jiang;LI Zhi-li(Department of Biophysics and Structural Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences&School of Basic Medicine,Peking Union Medical College,Beijing 100005,China;Department of Ophthalmology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100730,China;College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China)

机构地区：[1]中国医学科学院基础医学研究所,北京协和医学院基础学院,北京100005 [2]中国医学科学院北京协和医学院北京协和医院眼科,北京100730 [3]北京大学化学与分子工程学院,北京100871

出　　处：《质谱学报》2024年第6期793-799,I0003,共8页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金(21976005);中国医学科学院创新工程项目(2022-I2M-1-012);京津冀基础研究合作专项(J200006)。

摘　　要：糖尿病视网膜病变(diabetic retinopathy,DR)是糖尿病最常见的微血管并发症之一,也是造成全球工作年龄人群失明的主要原因。早期DR可通过控制血糖防止视力受损,晚期DR会导致不可逆转的视力丧失。因此,筛选早期DR诊断标志物对预防DR至关重要。前期研究发现,血清补体C3与DR的发生发展密切相关。本研究以C18为补体C3完整糖肽的富集材料,采用高性能质谱技术检测其糖肽,结合生物学统计方法,发现随着DR进展,补体C3半乳糖修饰水平呈先升高后降低趋势。受试者工作特征分析(receiver operating characteristic,ROC)结果表明,补体C3糖基化修饰具有良好的诊断效果,其诊断精度(area under curve, AUC)可达0.761。Diabetic retinopathy(DR),a common microvascular complication,is the leading cause of visual impairment among the working-age population.Late-stage DR can lead to irreversible vision loss,whereas vision impairment can be prevented by controlling blood sugar in a normal level in the early stage of DR.Notably,as early-stage DR is typically asymptomatic,the majority of DR patients are diagnosed at an advanced stage,which causes a great burden to the patients.Therefore,the development of accurate and efficient biomarkers for the early diagnosis of DR is essential for the prevention of DR.In recent decades,a large number of studies have found that serum complement C3 is closely associated with the development of DR.In this study,a method for the detection of complement C3 by mass spectrometry was established by optimizing the enrichment conditions of glycopeptides using C18 materials.It was found that among volunteers with different stages of DR,the level of complement C3 galactose modification showed a tendency to rise first and then go down with the development of DR.The difference in complement C3 glycosylation was used for the diagnosis of different stages of DR,yielding an area under curve(AUC)of up to 0.761.

关 键 词：糖尿病视网膜病变 补体C3 糖基化修饰 生物标志物 

分 类 号：O657.63[理学—分析化学]