产褐黄色链霉菌HEBRC45958菌株防控番茄棒孢叶斑病研究  

Study on Control Effect of Streptomyces phaeoluteichromatogenes HEBRC45958 Strain on Corynespora Leaf Spot of Tomato

作　　者：黄大野 余志斌[2] 万中义 杨丹 李金萍 曹春霞 HUANG Daye;YU Zhibin;WAN Zhongyi;YANG Dan;LI Jinping;CAO Chunxia(National Biopesticide Engineering Technology Research Center,Key Laboratory of Microbial Pesticides of Ministry ofAgriculture and Rural Affairs,Hubei Biopesticide Engineering Research Center,Wuhan 430064,China;Wuhan Business andTrade Vocational,Wuhan 430205,China;Beijing Plant Protection Station,Beijing 100029,China)

机构地区：[1]湖北省生物农药工程研究中心,国家生物农药工程技术研究中心,农业农村部微生物农药创制重点实验室,武汉430064 [2]武汉商贸职业学院,武汉430205 [3]北京市植物保护站,北京100029

出　　处：《中国农业科技导报》2024年第11期136-142,共7页Journal of Agricultural Science and Technology

基　　金：湖北省重点研发计划项目(2021BGD011);湖北省农业科技创新中心创新团队项目(2021-620-000-001-027)。

摘　　要：为明确产褐黄色链霉菌HEBRC45958菌株对番茄棒孢叶斑病的防控效果,采用平板对峙培养结合光学显微镜和扫描电镜观察确定HEBRC45958离体抑菌活性,并测定其产铁载体能力和水解酶活性。同时采用盆栽试验确定HEBRC45958发酵液对番茄棒孢叶斑病防控效果。结果表明,对峙培养条件下HEBRC45958对多主棒孢的抑菌率为52.65%。扫描电镜下观察,与对照相比,HEBRC45958处理后多主棒孢菌丝发生扭曲和破裂,透射电镜下观察,多主棒孢细胞膜和细胞壁距离增加。HEBRC45958菌株能产生淀粉酶、纤维素酶、几丁质酶和嗜铁素。盆栽试验结果表明,HEBRC45958发酵液对番茄棒孢叶斑病的防效为100%,具有较好的生防应用前景。In order to clarify the control effect of Streptomyces phaeoluteichromatogenes strain HEBRC45958 against Corynespora leaf spot of tomato,the antifungal activity was determined in vitro by using dual culture assay combined with microscope and scanning electron microscope observation.The siderophore production and the activities of hydrolases were also determined.The control effect of the fermentation broth of HEBRC45958 strain on Corynespora leaf spot of tomato was determined in pots.The results showed that the inhibition rate HEBRC45958 on Corynespora cassiicola was 52.65%in the dual culture.Compared with control,the mycelium treated with HEBRC45958 strain was distorted and cracked by scanning microscopy,and the space between the cell wall and cell membrane was widened by transmission electron microscopy.The HEBRC45958 strain could produce amylase,cellulase,chitinase and siderophore.The control effect of fermentation broth against Corynespora leaf spot of tomato was 100%in pot experiment,which showed that HEBRC45958 strain had a good prospect of biocontrol application for Corynespora leaf spot of tomato.

关 键 词：产褐黄色链霉菌 番茄 多主棒孢 防效 

分 类 号：S476[农业科学—农业昆虫与害虫防治]