忍冬功能基因挖掘研究进展  

Progress on the mining of functional genes of Lonicera japonica

作　　者：孙楠 黄璐瑶 杨盛 李佳[1] 侯丛哲 刘振华 Nan Sun;Luyao Huang;Sheng Yang;Jia Li;Congzhe Hou;Zhenhua Liu(Department of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Laboratory Center,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东中医药大学实验中心,济南250355

出　　处：《遗传》2024年第11期920-936,共17页Hereditas（Beijing)

基　　金：泰山学者工程专项(编号:tsqn202211135);山东省高等学校青创人才引育计划“中药功能基因挖掘及基因组学研究创新团队”;山东省重点研发计划(乡村振兴科技创新提振行动计划)项目(编号:2022TZXD0036)资助。

摘　　要：忍冬(Lonicera japonica Thunb.)为忍冬科忍冬属半常绿缠绕藤本植物,其干燥花蕾或待开放的花,即金银花,作为一种高值大宗中药材,同时亦具有药食两用的特性。目前金银花市场需求旺盛,但生产端忍冬新品种的选育技术相对落后,亟需引入现代育种技术。随着忍冬基因组学的发展,越来越多的功能基因被陆续鉴定,为忍冬分子育种积累了丰富的基因资源。本文系统总结了忍冬基因组学、功能基因挖掘以及遗传转化体系等方面的研究进展。针对目前忍冬功能基因研究和品质育种存在的问题和不足,本文指出要建立忍冬种质资源库、突变体库和高效遗传转化体系,需要深入开展其功能基因挖掘和鉴定工作,开发与忍冬功能基因密切连锁的分子标记,为培育忍冬优良品质突破性品种奠定基础。Lonicera japonica Thunb.is a semi-evergreen climbing shrub belonging to the Caprifoliaceae family,whose dried flower buds or flowers on the verge of blooming are known as Jin Yin Hua in traditional Chinese medicine.This plant is not only a high-value and widely used medicinal material but also possesses characteristics that make it suitable for both medicinal and culinary purposes.Currently,there is a robust market demand for Jin Yin Hua,yet the breeding technology for new varieties of Lonicera japonica lags behind,necessitating the integration of modern breeding techniques.With the advancement of genomics in Lonicera japonica,an increasing number of functional genes have been identified,amassing a rich reservoir of genetic resources for molecular breeding of this species.In this review,we summarize the progress in Lonicera japonica genomics,functional gene mining,and the establishment of genetic transformation systems.In light of the existing challenges and deficiencies in the research of functional genes and quality breeding of Lonicera japonica,it is imperative to establish a germplasm resource bank,a mutant library,and an efficient genetic transformation system for this plant.Intensive research into the mining and identification of functional genes should be conducted,and molecular markers closely linked to the functional genes of Lonicera japonica should be developed.This will lay a foundational basis for the identification and cultivation of breakthrough varieties with superior qualities in Lonicera japonica.

关 键 词：忍冬 功能基因 基因挖掘 品质育种 

分 类 号：S567.79[农业科学—中草药栽培]