中药芳香疗法对应激性失眠大鼠HPA轴相关激素水平影响的研究  

Study on the Effects of Aromatherapy on HPA Axis-Related Hormone Levels in Rats with Stress-Induced Insomnia

作　　者：何小英 贺荣[2] 张左芹 江雪菱 张玉萍[3] 杨丽 方丽波 HE Xiao-ying;HE Rong;ZHANG Zuo-qin;JIANG Xue-ling;ZHANG Yu-ping;YANG Li;FANG Li-bo(Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;Yunnan Hospital of Traditional Chinese Medicine,Kunming 650021,China;Yunnan Institute of Traditional Chinese Materia and Medica,Kunming,650223,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]云南省中医医院,云南昆明650021 [3]云南省中医中药研究院,云南昆明650223

出　　处：《云南中医中药杂志》2024年第10期70-76,共7页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：云南省科技厅-云南中医药大学联合专项面上项目(2019FF002-066)。

摘　　要：目的探讨芳香疗法对失眠模型大鼠下丘脑-垂体-肾上腺(HPA)轴功能的影响。方法将大鼠(除空白对照组)采用限制性制动方法造模,选取造模成功的心理应激性失眠大鼠30只,按体质量采用随机数字分组,分为模型组、芳香疗法组、舒乐安定组三组,每组各10只,未造模鼠10只为“空白对照组”。模型组和空白对照组常规饲养,不治疗;芳香疗法组予中药芳香精油雾化吸入治疗;舒乐安定组予以腹腔内注射舒乐安定,连续治疗14 d。第14天给药结束后,观察并记录各组大鼠情况。通过昼夜活动量测试和高架十字迷宫测试评价大鼠行为学改变,ELISA法测定各组大鼠血清ACh、5-HT、CRH、ACTH、CORT激素水平。结果与空白对照组比较,模型组大鼠昼夜持续活动时间、开臂次数百分比(OE%)减少、ACh激素水平降低(P<0.001);开臂滞留时间百分比(OT%)、高架十字迷宫运动距离延长、5-HT、CRH、ACTH、CORT浓度水平明显增加(P<0.001)。与模型组比较,芳香疗法组、舒乐安定组大鼠昼夜持续活动时间、OE%、ACh激素水平明显增加;OT%、高架十字迷宫运动距离减少,5-HT、CRH、ACTH、CORT激素水平明显降低(P<0.001);与舒乐安定组比较,芳香疗法组大鼠昼夜持续活动时间、高架十字迷宫运动距离缩短,ACh、CRH激素水平降低(P<0.05);而OE%、OT%增加,5-HT、ACTH、CORT激素水平较高(P<0.05)。结论经限制性制动束缚建立应激性失眠大鼠模型可使模型大鼠在行为方面表现出以高架十字迷宫OE%降低、运动距离明显增加为特点的睡眠障碍、焦虑特征,失眠模型大鼠血清5-HT、CRH、ACTH、CORT激素水平高于空白对照组,HPA轴功能亢进。芳香疗法可缩短应激性失眠大鼠开放臂活动时间和运动距离,通过降低5-HT、CRH、ACTH、CORT激素水平,进而抑制HPA轴兴奋性而发挥治疗作用。Objective:To study the effects of aromatherapy on hypothalamic-pituitary-adrenal(HPA)axis function in a rat model of insomnia.Methods:A rat model of stress-induced insomnia was established using a restricted immobilization method,excluding the blank control group.Thirty successfully modeled rats were randomly divided into three groups based on body weight:the model group,aromatherapy group,and diazepam group,10 rats each group.Another 10 non-modeled rats served as the blank control group.The model and blank control groups were housed normally without treatment,while the aromatherapy group received TCM essential oil aerosol inhalation therapy.The diazepam group was treated with intraperitoneal injections of diazepam for 14 consecutive days.After the final treatment on the 14th day,the rats'behaviors were observed and recorded.Behavioral changes were evaluated through light-dark activity tests and elevated cross maze tests.Serum levels of acetylcholine(ACh),serotonin(5-HT),corticotropin-releasing hormone(CRH),adrenocorticotropic hormone(ACTH),and cortisol(CORT)were measured using ELISA.Results:Compared with the blank control group,the model group showed a reduction in continuous day-night activity time,percentage of open arm entries(OE%),and ACh levels(P<0.001),while the percentage of time spent in the open arms(OT%)and distance traveled in the elevated cross maze increased significantly,along with elevated serum levels of 5-HT,CRH,ACTH,and CORT(P<0.001).Compared with the model group,the aromatherapy and diazepam groups showed significant increases in continuous day-night activity time,OE%,and ACh levels,while OT%,distance traveled in the maze,and levels of 5-HT,CRH,ACTH,and CORT decreased significantly(P<0.001).Compared with the diazepam group,the aromatherapy group had shorter continuous day-night activity time and maze distance,with lower ACh and CRH levels(P<0.05),but higher OE%,OT%,and 5-HT,ACTH,and CORT levels(P<0.05).Conclusion:The restricted immobilization method used to establish a rat model of stress-indu

关 键 词：芳香疗法 应激性失眠 失眠 HPA轴 

分 类 号：R285.5[医药卫生—中药学]