彝药小红参对LPS诱导大鼠乳腺炎的作用机制及研究  

Mechanism and Study of Yi Medicine Xiao Hongshen on LPS-Induced Mastitis in Rats

作　　者：段蓉 李本发 陈婉怡 黄敏 DUAN Rong;LI Ben-fa;CHEN Wan-yi;HUANG Min(Yunnan Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming 650021,China;Yunnan University of Traditional Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南省中医医院/云南中医药大学第一附属医院,云南昆明650021 [2]云南中医药大学,云南昆明650500

出　　处：《云南中医中药杂志》2024年第10期76-80,共5页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：云南省教育厅科学研究基金项目(2023Y0476)。

摘　　要：目的探究彝药小红参对大鼠乳腺炎的影响。方法小红参汤组成:小红参30 g,制成水煎液。将50只SD大鼠随机分为5组,分别为A(空白对照组)、B(LPS模型组)、C(LPS+2.5mg/kg小红参汤低剂量组)、D(LPS+5mg/kg小红参汤中剂量组)、E(LPS+10mg/kg小红参汤高剂量组)。将分娩SD大鼠与仔鼠分隔1h后开始灌胃,LPS+小红参汤低、中、高剂量组予小红参汤稀释液(0.125、0.25、0.5mg/mL)灌胃;空白对照组和LPS模型组予生理盐水灌胃,每日1次,共5 d。末次灌胃1h后造大鼠LPS乳腺炎模型,空白对照组灌注生理盐水。间隔12h后对LPS+小红参汤低、中、高剂量组继予等量小红参汤灌胃,空白组和模型组继予等量生理盐水灌胃。造模24h后取第4对乳腺组织HE染色观察;选择Elisa测定大鼠血清中IL-1、IL-6与TNF-α的含量。结果模型组乳腺组织损伤严重,血清中IL-1、IL-6、TNF-α的含量升高;实验组乳腺组织损伤减轻,血清中IL-1、IL-6、TNF-α的含量降低。结论彝药小红参能减轻大鼠急性乳腺炎症,降低血清中的炎症因子。Objective:To study the effects of the Yi medicine Xiao Hongshen on rat mastitis.Methods:The decoction of Xiao Hongshen was prepared with 30g of Xiao Hongshen.Fifty SD rats were randomly divided into five groups:Group A(control),Group B(LPS model),Group C(LPS+low dose Xiao Hongshen decoction,2.5 mg/kg),Group D(LPS+medium dose Xiao Hongshen decoction,5 mg/kg),and Group E(LPS+high dose Xiao Hongshen decoction,10 mg/kg).After separating lactating SD rats from their pups for 1 hour,intragastric administration was initiated.Groups C,D,and E received diluted Xiao Hongshen decoction at concentrations of 0.125,0.25 and 0.5 mg/mL,respectively,while the control and model group received normal saline.This was administered once daily for 5 days.One hour after the last administration,the LPS-induced mastitis model was created for all groups except the control,which received saline.After 12 hours,groups C,D,and E continued to receive the corresponding doses of Xiao Hongshen decoction,while the control and model groups continued to receive saline.At 24 hours post-modeling,the fourth pair of mammary gland tissues were collected for histological examination using HE staining.ELISA was employed to measure serum levels of IL-1,IL-6,and TNF-α.Results:The model group exhibited severe mammary tissue damage,along with elevated serum levels of IL-1,IL-6,and TNF-α.In contrast,the treatment groups showed reduced tissue damage and lower serum levels of IL-1,IL-6,and TNF-α.Conclusion:Xiao Hongshen,a Yi medicine,can alleviate acute mastitis inflammation in rats and reduce the levels of inflammatory factors in the serum.

关 键 词：急性乳腺炎 LPS 彝药小红参 

分 类 号：R271.44[医药卫生—中医妇科学]