经典名方羌活胜湿汤中12种成分的LC-MS/MS定量分析  

Simultaneous determination of 12 components in Qianghuo Shengshi Decoction by liquid chromatography-tandem mass spectrometry

作　　者：李鑫[1,2,3] 王敏[1,2,3] 邢斌 贾欣陶 刘志东[1,2,3] LI Xin;WANG Min;XING Bin;JIA Xintao;LIU Zhidong(State Key Laboratory of Component-based Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique,Ministry of Education,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Haihe Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学,组分中药国家重点实验室,天津301617 [2]天津中医药大学,现代中药发现与制剂技术教育部工程研究中心,天津301617 [3]天津中医药大学,现代中医药海河实验室,天津301617

出　　处：《天津中医药大学学报》2024年第9期780-786,共7页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：现代中医药海河实验室科技项目(22HHZYSS00005);国家中医药管理局青年岐黄学者项目。

摘　　要：[目的]建立液相色谱-质谱联用(LC-MS/MS)分析方法用于测定羌活胜湿汤颗粒中5-O-甲基维斯阿米醇苷、紫花前胡苷、蔓荆子黄素、羌活醇、蛇床子素、二氢欧山芹醇当归酸酯、异甘草苷、甘草苷、绿原酸、异甘草素、甘草素、阿魏酸12种成分的含量。[方法]色谱柱为ACQUITY UPLC BEH C18柱(2.1 mm×100 mm,1.7μm),流动相为0.1%的甲酸水溶液和乙腈,梯度洗脱,流速为0.3 mL/min,柱温为30℃,进样量为5μL。采用电喷雾离子源(ESI)、多反应监测模式(MRM)进行正负离子同时扫描。[结果]羌活胜湿汤颗粒中12种成分在线性范围内线性关系良好,精密度、重复性、稳定性及加样回收率试验均满足要求。[结论]实验建立的含量测定方法高效、稳定、可靠,可用于羌活胜湿汤颗粒的质量控制研究。[Objective]A liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was established for the simultaneous determination of 12 components in Qianghuo Shengshi Decoction granules,including 5-O-methylvisammioside,nodakenin,casticin,notopterol,osthole,columbianadin,isoliquiritin,liquiritin,chlorogenic acid,isoliquiritigenin,liquiritigenin,and ferulic acid.[Methods]The chromatography was performed on an ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)with a gradient elution of 0.1%formic acid aqueous solution and acetonitrile as mobile phase at the flow rate of 0.3 mL/min.The column temperature was 30℃and the injection volume was 5μL.The samples were detected by an electron spray ionization(ESI)in both positive and negative ion modes using the multiple reaction monitoring(MRM)mode.[Results]The linearity of the 12 components in Qianghuo Shengshi Decoction granules was found to be good within their linear ranges.The results of the precision,reproducibility,stability tests,and recoveries met the requirements.[Conclusion]The method established in this experiment is efficient,stable,and reliable,and can be used for the quality control of Qianghuo Shengshi Decoction granules.

关 键 词：羌活胜湿汤 液相色谱-质谱联用 含量测定 中药多成分分析 

分 类 号：R284.1[医药卫生—中药学]