Bacteriophages assisted bacteria to facilitate soil multifunctionality under organochlorine pesticide contamination  

作　　者：ZHAO DuoKai CHAO HuiZhen BALCAZAR Jose Luis WU ShiMao ZHENG XiaoXuan WU YunLing YUAN ShuJian SUN MingMing HU Feng 

机构地区：[1]Soil Ecology Lab,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization and Jiangsu Key Laboratory for Solid Organic Waste Utilization,Nanjing Agriculture University,Nanjing 210095,China [2]J.F.Blumenbach Institute of Zoology and Anthropology,University of Göttingen,Untere Karspüle 2,Göttingen 37073,Germany [3]Catalan Institute for Water Research(ICRA),Girona 17003,Spain [4]University of Girona,Girona 17004,Spain [5]College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310085,China

出　　处：《Science China(Technological Sciences)》2024年第10期3116-3128,共13页中国科学（技术科学英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.42077106,42277115,and 42177113);the Key R&D Project of Jiangsu Province(Modern Agriculture,Grant No.BE2022322);the Fundamental Research Funds for the Central Universities(Grant No.YDZX2023023).

摘　　要：As the most abundant living entities in the environment,viruses have been well recognized as crucial members in sustaining biogeochemical cycling.However,the significance of viruses in soil ecosystem multifunctionality remains under-explored.In this study,we used metagenomics and meta-viromics analysis to investigate the role of soil viruses in soil ecosystem functions under heavy,light,and no organochlorine pesticides(OCPs)contamination.In the three types of soil samples collected,lightcontaminated soils supported the highest level of multifunctionality,followed by heavy-contaminated soils and clean soils.Additionally,our results revealed a positive correlation between bacterial community evenness and multifunctionality index(p<0.05).Dominant bacterial species with biodegradation and stress resistance advantages exhibited higher abundance in OCPaffected soils,potentially playing a core functional supporting role.Furthermore,our results indicated that the species richness and diversity of bacteriophages were positively correlated with multifunctionality(p<0.05)in OCP-affected soils.Bacteriophages in OCP-affected soils regulate host metabolism and enhance soil ecosystem multifunctionality by infecting functional bacterial hosts and encoding AMGs related to soil element cycling.Our findings emphasize the potential effect of phages on ecosystem multifunctionality in contaminated soil,suggesting that phages may serve as contributors to soil ecology beyond bacteria and other microorganisms.Therefore,in polluted or constrained soils,further research could potentially translate phage communities and related ecological processes into artificial methods for application in soil pollution remediation or ecological restoration.

关 键 词：soil ecosystem multifunctionality viruses auxiliary metabolic genes organochlorine pesticides contamination 

分 类 号：X53[环境科学与工程—环境工程]