Peroxidase-like nanozyme based microenvironment-responsive injectable hydrogel for bacteria-infected wound healing  

作　　者：ZHOU Wei LI XiaNan TANG MengCheng PAN LingFeng WANG BeiBei ZHANG Hao ZHANG Han WANG ShiBo KONG XiangDong 

机构地区：[1]Institute of Smart Biomaterial Materials,School of Materials Science and Engineering,Zhejiang University of Science&Technology,Hangzhou 310018,China [2]Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering,Zhejiang University of Science&Technology,Hangzhou 310018,China

出　　处：《Science China(Technological Sciences)》2024年第10期3247-3260,共14页中国科学（技术科学英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.52102353);the Natural Science Foundation of Zhejiang Province(Grant No.LQ22E030018);the Fundamental Research Funds of Tongxiang Research Institute of Zhejiang Sci-Tech University(Grant No.TYY202212);the Key Research&Development Program of Zhejiang Province(Grant Nos.2021C01180 and 2019C04020).

摘　　要：Hydrogel stands out as one of the most attractive wound dressings due to its excellent moisturizing properties and capacity to absorb wound exudates.However,conventional hydrogel dressings often lack responsiveness to the microenvironment,merely acting as protective barriers for the wound.Consequently,they exhibit limited effectiveness in preventing infection and facilitating wound repair.To address these problems,we have developed a multifunctional injectable hydrogel,CF/MS@HG,based on peroxidase-like nanozymes,aiming at rapidly healing bacterial-infected wounds.The hydrogel is mainly composed of oxidized sodium alginate,aminated gelatin,and polylysine,encapsulating MIL-101(CuFe)NPs(CF)and manganese selenide nanoparticles(MnSe_(2) NPs,or MS NPs).After injection,the complex rapidly gelatinizes at the infected wound site through a Schiff base reaction.In vitro experiments have demonstrated the hydrogel’s strong adhesion and self-healing capabilities.Moreover,CF exhibiting peroxidase(POD)-like activity,catalyzes in situ hydrogen peroxide(H_(2)O_(2))to generate highly toxic hydroxyl radicals(·OH)within the wound microenvironment,inducing oxidative damage to bacteria.Meanwhile,MS decomposes into H2Se in the slightly acidic wound microenvironment,disrupting bacterial metabolism and inhibiting proliferation.The addition of polylysine further enhances the hydrogel’s antibacterial properties.In vivo experiments have shown that the hydrogel exhibits excellent biological safety and significantly promotes wound healing.This multifunctional smart hydrogel holds great promise for the treatment of bacterial-infected wounds.

关 键 词：peroxidase-like nanozyme manganese selenide injectable hydrogel bacterial infection wound healing 

分 类 号：TQ427.26[化学工程] R318.08[医药卫生—生物医学工程]