可提高漆酶酶活的载锰活性炭-纤维素复合吸附剂的制备及应用  

Preparation and Application of Manganese-loaded Activated Carbon-Cellulose Composite Adsorbent for Enhancing Laccase Enzyme Activity

作　　者：李娜 孟祥锐 彭传博 于方睿 张健 盛雪茹 平清伟 LI Na;MENG Xiangrui;PENG Chuanbo;YU Fangrui;ZHANG Jian;SHENG Xueru;PING Qingwei(School of Light Industry and Chemical Engineering,Dalian Polytechnic University,Dalian,Liaoning Province,116034)

机构地区：[1]大连工业大学轻工与化学工程学院,辽宁大连116034

出　　处：《中国造纸学报》2024年第3期38-44,共7页Transactions of China Pulp and Paper

基　　金：辽宁省教育厅面上项目(LJKMZ20220885);生物基材料与绿色造纸国家重点实验室开放基金项目(GZKF202225);天津市制浆造纸重点实验室开放基金项目(202106)。

摘　　要：利用废弃的载锰活性炭与纤维素,通过溶剂再生法制备了一种可用于固定化漆酶、提高固定化漆酶酶活的载锰活性炭-纤维素复合吸附剂,并用其固定化漆酶制备漆酶-载体材料。研究结果表明,Mn^(2+)含量对漆酶-载体材料酶活有一定的影响,复合吸附剂中Mn^(2+)含量为1.5 mg/g时,漆酶-载体材料酶活最高;漆酶固定化的最佳工艺条件为固定化温度45℃、pH值=4,初始酶浓度6.5 g/L;在最佳固定化反应条件下,漆酶-载体材料的漆酶固定化率最高达48.24%;漆酶-载体材料的热稳定性和pH稳定性均得到明显增强;利用漆酶-载体材料降解双酚A,降解率达68%,重复使用5次后的双酚A相对降解率仍可达51%。A novel manganese-loaded activated carbon-cellulose composite adsorbent that could immobilize laccase and increase the enzyme activity was prepared by solvent regeneration method,using waste manganese-loaded activated carbon and cellulose,and further prepared the laccase-carrier material after immobilizing laccase.The results showed that the content of Mn^(2+)had a certain effect on the enzyme activity of the laccase-carrier material,and the enzyme activity of the laccase-carrier material was the highest when the content of Mn^(2+)in the composite adsorbent was 1.5 mg/g.The optimum process conditions for the immobilization of laccase were the immobilization temperature of 45℃,the pH value of 4,and the initial enzyme concentration of 6.5 g/L.The immobilization ratio of the laccase-carrier material was the highest to 48.24%under the optimum conditions of immobilization;the thermal and pH stabilities of the laccase-carrier material were significantly enhanced;the degradation rate of bisphenol A using the laccase-carrier material reached 68%,and the relative degradation rate of bisphenol A was still up to 51%after 5 times of reuse.

关 键 词：废弃载锰活性炭 纤维素基吸附剂 固定化漆酶 双酚A降解 

分 类 号：TS7[轻工技术与工程—制浆造纸工程]