Exploring the characteristics of microbial community and enzyme activity in the aged refuse landfill environment of Taiyuan,China  

作　　者：Fen HOU Junjie DU Yuzhou MENG Xihui WU 

机构地区：[1]Department of Land Resource Management,School of Public Administration,Shanxi University of Finance and Economics,Taiyuan 030006,China [2]College of Life Science,Shanxi Engineering Research Center of Microbial Application Technologies,Shanxi Normal University,Taiyuan 030031,ChinaTechnologies,Shanxi Normal University

出　　处：《Pedosphere》2024年第5期879-891,共13页土壤圈（英文版）

基　　金：financially supported by the Fundamental Research Program of Shanxi Province,China(Nos.202103021224263 and 202203021212493)。

摘　　要：Municipal solid waste landfill is the main disposal option for domestic garbage,in which microbial activities play an important role.However,despite the widespread practice of landfilling,the metagenomic microbial profiles of landfill sites remain poorly characterized.In this study,we used a combination of physicochemical analysis,ultraviolet-visible spectrophotometry,and high-throughput Illumina shotgun sequencing to systematically investigate the changes in soil enzyme activities,microbial community structure,and functional attributes in aged refuse collected from the Xingou Municipal Solid Waste Landfill in Taiyuan,China,with ordinary topsoil from an area within 5 km of the landfill as control soil.Except for neutral phosphatase(P=0.065),the activities of urease,laccase,dehydrogenase,sucrase,neutral protease,andβ-glucosidase were all significantly reduced(P<0.05)in the aged refuse compared with the control soil.Contrastingly,catalase activity was found to be significantly elevated in the aged refuse.Compared with the control soil,aged refuse was characterized by higher richness and diversity of microbial communities,as reflected by the higher values of community richness estimators(Chao 1 and ACE)and diversity indices(Shannon and Simpson).In total,186 phyla,4354 genera,and 34459 species were identified,with 132 phyla,1914 genera,and7369 species showing significantly different abundances between the aged refuse and the control soil.Actinobacteria and Acidobacteria were identified as the dominant phyla in the control soil,whereas Proteobacteria,Euryarchaeota(archaea),and Firmicutes were found to predominate in the aged refuse.Notably,Euryarchaeota and Methanoculleus were the major taxa detected in the aged refuse,but were almost completely absent in the control soil.Xenobiotic biodegradation and bacterial chemotaxis were the main functions of the microflora in the aged refuse,whereas the carbohydrate,amino acid,energy,and lipid metabolism pathways were significantly enriched in the control soil.Moreover,the age

关 键 词：functional attribute metabolism pathway municipal solid waste landfll resource utilization soil microflora 

分 类 号：X705[环境科学与工程—环境工程]