丹寿汤调节miR-744-3p降低内皮细胞损伤干预复发性流产的研究  

Danshou Decoction reduces endothelial cell damage by regulating miR-744-3p to relieve recurrent spontaneous abortion

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作  者:徐广立 李彦茹 李会娟[1] 张晨雨 王晶[1] 任姝晴 陈萍[1] XU Guang-li;LI Yan-ru;LI Hui-juan;ZHANG Chen-yu;WANG Jing;REN Shu-qing;CHEN Ping(The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou Henan 450000,China)

机构地区:[1]河南中医药大学第一附属医院,河南郑州450000

出  处:《时珍国医国药》2024年第12期2797-2803,共7页Lishizhen Medicine and Materia Medica Research

基  金:国家自然科学基金(82074167)。

摘  要:目的探讨丹寿汤调节微小RNA-744-3P(miR-744-3p)降低内皮细胞损伤干预复发性流产的作用及其潜在分子机制。方法收集复发性流产模型组和丹寿汤干预组小鼠胎盘组织,称重胚胎并计算胚胎吸收率,Real-time PCR检测胎盘组织中miR-744-3p的表达;转染miR-744-3p,模拟物(mimics)或抑制物(inhibitors)至人脐静脉内皮细胞中,通过CCK-8法、划痕实验和小管形成实验来检测miR-744-3p对内皮细胞增殖、迁移和成管能力的影响;利用Real-time PCR和Western blot技术检测miR-744-3p对TLR4表达的影响,并通过双荧光素酶报告基因实验来验证miR-744-3p对TLR4的靶向性。结果与模型组相比,丹寿汤治疗可显著抑制复发性流产小鼠胚胎吸收率并上调miR-744-3p表达;与对照组相比,过表达miR-744-3p可显著增强内皮细胞的增殖、迁移及成管能力,而抑制miR-744-3p时结果则与之相反;miR-744-3可通过靶向TLR4来抑制其表达。结论丹寿汤通过诱导miR-744-3p表达来靶向抑制TLR4的表达,从而降低内皮细胞损伤并缓解复发性流产疾病进程。Objective To observe the effect of Danshou Decoction on regulating miR-744-3p,reducing endothelial cell damage and alleviating recurrent spontaneous abortion and its potential molecular mechanism.Methods The placental tissues of mice in the recurrent spontaneous abortion model group and the Danshou Decoction group were collected,and then we weighed the embryo and calculated the embryo absorption rate.The expression of miR-744-3p in the tissues was detected by Real-time PCR.MiR-744-3p mimics or inhibitors were transfected into human umbilical vein endothelial cells.The effects of miR-744-3p on the proliferation,migration and tubule formation of endothelial cells were detected by CCK-8,scratch and tubule formation test.Real-time PCR and Western blot were used to detect the effect of miR-744-3p on TLR4 expression,and double luciferase reporter gene assay was used to verify the targeting of miR-744-3p to TLR4.Results Compared with the model group,Danshou Decoction could significantly inhibit the embryo absorption rate and up-regulate the expression of miR-744-3p in recurrent abortion mice.Overexpression of miR-744-3p significantly enhanced the proliferation,migration and tubular formation of endothelial cells compared with the control group,while inhibition of miR-744-3p showed the opposite results.MiR-744-3p can inhibit TLR4 expression by directly targeting TLR4.Conclusion Danshou decoction can inhibit the expression of TLR4 by inducing the expression of miR-744-3p,thus reducing endothelial cell damage and alleviating the process of recurrent spontaneous abortion.

关 键 词:复发性流产 丹寿汤 内皮细胞损伤 微小RNA-744-3p TOLL样受体4 

分 类 号:R285.6[医药卫生—中药学] R271.41[医药卫生—中医学]

 

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