磁铁矿作用下DvH阴极生物膜的响应及转录组分析  

Response and transcriptional analysis of DvH cathode biofilm with the effect of magnetite

作　　者：滕敏 朱曦 曾翠平 胡佳萍 刘广立 骆海萍 TENG Min;ZHU Xi;ZENG Cui-ping;HU Jia-ping;LIU Guang-li;LUO Hai-ping(Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology,School of Environmental Science and Engineering,Sun Yat-sen University,Guangzhou 510006,China;Shenzhen Institute of Synthetic Biology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China)

机构地区：[1]中山大学环境科学与工程学院,广东省环境污染控制与修复技术重点实验室,广东广州510006 [2]深圳合成生物学创新研究院,中国科学院深圳先进技术研究院,广东深圳518055

出　　处：《中国环境科学》2024年第11期6088-6095,共8页China Environmental Science

基　　金：国家自然科学基金资助项目(42077286);广东省自然科学基金资助项目(2023A1515011846)。

摘　　要：本研究通过构建Desulfovibrio vulgaris Hildenborough(DvH)生物阴极,探究磁铁矿纳米颗粒(MNPs)作用下其生物膜的响应机制.结果发现,相较于无MNPs添加组,MNPs介导下的DvH生物阴极的SO_(4)^(2-)-S~(2-)转化率由6.8%提升至37.9%,周期电荷量提升了11.5%.线性伏安扫描结果显示,在0~-0.81V电势范围内,MNPs添加组阴极电流均高于对照组,说明MNPs可以增强阴极生物膜电催化活性.DvH阴极生物膜的比较转录组结果表明,MNPs可通过促进与[FeFe]氢化酶、Hmc和ATP合成酶相关的基因的表达,促进DvH对氢气的利用和代谢;通过促进编码ATP硫酰化酶和腺苷酸硫酸还原酶这两种硫代谢关键酶的基因表达上调,提高SO_(4)^(2-)-S^(2)转化率;通过促进Flp/Tad菌毛组装和PEP-CTERM蛋白相关基因的表达上调,强化DvH在阴极的附着和成膜.研究结果可为微生物阴极电化学系统的高效构建提供新的理论依据.This study investigated the response mechanism of the biofilm by constructing a Desulfovibrio vulgaris Hildenborough(DvH)biocathode,under the influence of magnetite nanoparticles(MNPs).The results showed that the conversion rate of SO_(4)^(2-)to S^(2-)of the biocathode mediated by MNPs increased from 6.8%to 37.9%,compared to the group without MNPs.The results of the linear sweep voltammetry test revealed that,within the potential range of 0~-0.81V,the cathodic current observed in the group with MNPs was higher than that of the control group,suggesting that MNPs enhance the electrocatalytic activity of the cathodic biofilm.The comparative transcriptome analysis of DvH cathodic biofilm revealed that MNPs can facilitate hydrogen utilization in DvH by upregulating genes encoding[FeFe]hydrogenase,Hmc,and ATP synthase.In addition,genes encoding sulfate adenylyltransferase and adenylylsulphate reductase,both crucial enzymes in sulfur metabolism,were upregulated with MNPs addition,which contributed to an improved conversion rate of SO_(4)^(2-)-S^(2-).MNPs upregulated genes related to Flp/Tad pili assembly and PEP-CTERM protein,thereby augmenting the adhesion of DvH and promoting biofilm formation on the cathode.These findings contribute to a novel theoretical framework for the development of highly efficient microbial cathodic electrochemical systems.

关 键 词：微生物电化学系统 硫酸盐还原菌 生物阴极 纳米磁铁矿 转录组学 

分 类 号：X703.1[环境科学与工程—环境工程]