复杂碳源对生物膜系统磷富集及微生物群落的影响  

Effects of complex carbon sources on phosphorus enrichment and microbial communities in biofilm systems

作　　者：毕贞 付豪 王雪玲 袁艺娜 黄勇[1,2] BI Zhen;FU Hao;WANG Xue-ling;YUAN Yi-na;HUANG Yong(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009 [2]苏州科技大学,城市生活污水资源化利用技术国家地方联合工程实验室,江苏苏州215009

出　　处：《中国环境科学》2024年第11期6096-6104,共9页China Environmental Science

基　　金：国家自然科学基金资助项目(51938010)。

摘　　要：本研究参照城市污水中的组成配置模拟污水,在厌氧/好氧交替运行的生物膜反应器中,考察由VFAs、糖类和氨基酸组成的复杂碳源对生物膜磷富集效果以及微生物群落代谢特征、群落特征的影响.结果表明,在复杂碳源条件下,通过调控厌氧段水力停留时间、好氧段p H值和DO等参数,生物膜对复杂碳源的利用率达到72.5%,磷富集液浓度超过50mg/L,总磷和总氮去除率分别为99.8%和76.5%.在利用复杂碳源时,聚磷生物膜可能利用氨基酸作为胞内储能物质进行磷代谢,以弥补胞内PHA和糖原合成降低所引起的能量不足,从而维持系统良好的磷去除与富集效果.在生物膜吸/释磷过程中生物膜内有机磷含量显著增加/降低,而聚磷含量变化较小,表明PAOs的磷代谢由无机的聚磷代谢模式转变为有机磷代谢模式.以复杂碳源为底物时,具有水解和发酵功能的微生物丰度显著提高,可为PAOs代谢提供小分子有机碳,进而维持系统良好的磷去除与富集效果.研究结果表明:生物膜系统具有利用城市污水复杂碳源同步实现磷富集与总氮去除双重目标的潜力.In this study,the effects of complex carbon sources on the performance of phosphorus enrichment and characteristics of microbial community were investigated in an anaerobic/aerobic alternatively operational biofilm reactor by using synthetic sewage wastewater containing VFAs,glucose and amino acid.The results showed that the utilization efficiency of complex carbon source reached 72.5%by adjusting the parameters of anaerobic hydraulic retention time,aerobic pH and DO concentration.The removal efficiency of total phosphorus and total nitrogen were 99.8%and 76.5%,respectively,with the concentration of phosphorus enrichment solution exceeding 50mg/L.The utilization complex carbon source changed the energy metabolism pathway of biofilm.Specifically,amino acids may participate in phosphorus metabolism as intracellular energy storage substances to compensate for the energy deficiency caused by PHA and glycogen,thus maintaining a good phosphorus removal and enrichment performance of the biofilm system.Meanwhile,the content of intracellular organophosphorus(OP)fluctuated with the activity of phosphorus uptake and release,whereas the content of inorganic poly-phosphorus(IP)changed a little.This phenomenon indicated that the phosphorus metabolism transferred from the IP pathway to the OP pathway.The abundance of hydrolytic bacteria and fermentative bacterium in biofilm increased significantly,which could provide small molecular organic carbon for phosphorus-accumulating bacteria(PAOs)metabolism,thus contribute the satisfactory performance of phosphorus removal and enrichment.The conclusion of present study exhibited the potential of biofilm system to simultaneously realize the phosphorus enrichment and total nitrogen removal via using complex carbon sources in sewage wastewater.

关 键 词：进水碳源 生物膜 磷富集 城市污水资源化 胞内有机磷 

分 类 号：X703[环境科学与工程—环境工程]