聚丙烯纳米塑料与有机磷阻燃剂联合暴露对斑马鱼发育和神经毒性的影响  

Combined effects of polypropylene nanoplastics and organophosphorus flame retardants on zebrafish development and neurotoxicity

作　　者：周鑫 万可维 倪安煜 方露 包君婷 陈嘉怡 王慧利 钱秋慧 王泽君 闫瑾 ZHOU Xin;WAN Ke-wei;NI An-yu;FANG Lu;BAO Jun-ting;CHEN Jia-yi;WANG Hui-li;QIAN Qiu-hui;WANG Ze-jun;YAN Jin(The school of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009

出　　处：《中国环境科学》2024年第11期6484-6494,共11页China Environmental Science

基　　金：国家自然科学基金资助项目(22106118)。

摘　　要：以斑马鱼为模式生物,探究了2-乙基己基二苯基磷酸酯(EHDPP)暴露以及与聚丙烯纳米塑料(PP-NPs)联合暴露的毒性效应及致毒机制.结果表明,EHDPP对120hpf斑马鱼幼鱼急性毒性LC50值为2240μg/L.250、500μg/LEHDPP急性暴露引发斑马鱼发育延缓和畸形,抑制幼鱼自主游动活力,降低其对周围环境的感知灵敏度;在分子水平上,50、125、250μg/LEHDPP较对照组,均显著降低斑马鱼幼鱼的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,导致活性氧(ROS)在体内过度蓄积,丙二醛(MDA)水平上升,进而诱发机体炎症,加快细胞凋亡.尽管PP-NPs对斑马鱼幼鱼的毒性有限,100μg/L PP-NPs与EHDPP的联合暴露使得其LC50值降为494.6μg/L,显著增强EHDPP的毒性,并降低了EHDPP对上述效应的阈值,致使低浓度EHDPP即引起显著的发育和神经毒性.本研究强调纳米塑料与有机污染物共存时会产生协同毒性作用,对于理解微塑料与有机污染物在实际环境共存的相关风险具有重要意义.Using zebrafish as a model organism,this study investigated the toxic effects and the relevant mechanisms for the exposure to 2-ethylhexyl diphenyl phosphate(EHDPP),as well as the co-exposure to it with polypropylene nanoplastics(PP-NPs).The results indicated that the acute toxicity LC50value of EHDPP for zebrafish at 120hours post-fertilization(hpf)was 2240μg/L.Acute exposure to 250 and 500μg/L EHDPP resulted in developmental delays and deformities in zebrafish,inhibited their swimming activity and reduced the sensitivity to environmental stimuli.At the molecular level,EHDPP concentrations of 50,125,and 250μg/L significantly decreased the activities of superoxide dismutase(SOD)and catalase(CAT)compared to the control group,leading to excessive accumulation of reactive oxygen species(ROS)and increased malondialdehyde(MDA)levels,which in turn induced inflammation and accelerated cell apoptosis.Although PP-NPs showed limited toxicity to zebrafish,the co-exposure to 100μg/L PP-NPs and EHDPP reduced the LC50 value to 494.6μg/L,significantly enhancing the toxicity of EHDPP and lowering the threshold for its adverse effects,resulting in noticeable developmental and neurotoxic effects at lower concentrations.This study highlights the synergistic toxic effects that can arise from the coexistence of nanoplastics and organic pollutants,providing important insights into the risks associated with microplastics and organic contaminants in real environmental scenarios.

关 键 词：EHDPP 斑马鱼 神经毒性 氧化应激 炎性响应 

分 类 号：X503.2[环境科学与工程—环境工程]