A1型反应性星形胶质细胞在幼鼠单次术后认知功能损害中的影响  

The effect of A1 astrocytes on cognitive impairment after one‑time anesthesia and surgery in infancy mice

作　　者：张月晗 涂志祎 王妍妍 吕雪阳 赵璇 Zhang Yuehan;Tu Zhiyi;Wang Yanyan;Lyu Xueyang;Zhao Xuan(Tongji University School of Medicine,Shanghai 200092,China;Department of Anesthesiology,Shanghai Tenth People's Hospital Affiliated to Tongji University,Shanghai 200072,China)

机构地区：[1]同济大学医学院,上海200092 [2]同济大学附属上海市第十人民医院麻醉科,上海200072

出　　处：《国际麻醉学与复苏杂志》2024年第9期905-910,共6页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金(82201323)。

摘　　要：目的观察A1型反应性星形胶质细胞在幼鼠单次术后认知功能损害中的影响。方法SPF级健康C57BL/6孕鼠8只,孕18 d。出生后8日龄幼鼠采用随机数字表法分为2组(每组27只):对照组和麻醉手术组。对照组幼鼠给予40%氧,麻醉手术组幼鼠给予40%氧、3%七氟醚麻醉,麻醉后行剖腹探查手术。两组建立麻醉手术模型后1.5 h各取3只幼鼠麻醉后处死,取脑组织做冰冻切片,免疫荧光染色法检测海马区域胶质纤维酸性蛋白(GFAP)及补体C3(C3)的表达情况;小鼠30日龄时两组各取6只麻醉后处死并取海马组织,免疫印迹法检测突触后致密蛋白(PSD)95含量。其余每组各18只幼鼠于30~36日龄时进行Morris水迷宫实验(定位航行试验、空间探索实验),记录逃避潜伏期和穿越平台次数。结果与对照组比较,麻醉手术组幼鼠在Morris水迷宫定位航行实验的第6天和第7天逃避潜伏期均延长(均P<0.05),第7天在Morris水迷宫空间探索实验中穿越平台次数减少(均P<0.05)。与对照组比较,麻醉手术组海马区域C3/GFAP表达升高,即海马区域星形胶质细胞向A1型极化比例较高(P<0.05),PSD95含量较低(P<0.05)。结论幼鼠单次麻醉手术后即可产生认知功能损害。星形胶质细胞向A1型过度极化,导致海马区域PSD95水平降低。Objective To observe the effect of A1 astrocytes on cognitive impairment in infancy mice after single‑time anes‑thesia and surgery.Methods A total of eight healthy C57BL/6 pregnant mice of SPF grade were gestated for 18 d.The 8‑day‑old in‑fancy mice were randomly divided into two groups(n=27),according to the random number table method:a control group and a single‑time anesthesia and surgery group.The infant mice of control group were given 40%oxygen,while the single‑time anesthesia and surgery group were given 40%oxygen and 3%sevoflurane anesthesia.After anesthesia,the infant mice of single‑time anesthesia and surgery group underwent an exploratory surgery.Three infant mice in each group were killed after anesthesia 1.5 h after establish‑ing the single‑time anesthesia and surgery model.Brain tissue was taken for frozen sections,and the expression of glial fibrillary acidic protein(GFAP)and complement C3(C3)in the hippocampus was detected using immunofluorescence staining;at 30 d of age,6 mice in each group were sacrificed after anesthesia to the hippocampus,and the content level of postsynaptic density protein(PSD)95 was de‑tected by Western blot.The remaining 18 infant mice were subjected to Morris water maze experiments at the age of 30-36 d,recording the escape latency and the platform crossing times.Results Compared to mice in the control group,the escape latency of mice in the Morris water maze navigation experiment was significantly prolonged(all P<0.05)in the anesthesia surgery group on the day 6 and 7,and the number of platform crossings in the Morris water maze spatial exploration experiment was significantly reduced(P<0.05)on the day 7.Compared to the mice in control group,the C3/GFAP levels of mice in the hippocampal region were significantly increased in the anesthesia surgery group,indicating that the hippocampal region astrocytes were more polarized towards type A1(P<0.05),and the PSD95 expression level was significantly reduced(P<0.05).Conclusions Single anesthesia surgery in

关 键 词：星形胶质细胞 七氟醚 单次手术 认知功能损害 幼鼠 

分 类 号：R614[医药卫生—麻醉学]