维生素D在改善七氟醚麻醉致老年小鼠认知功能障碍中的作用  

The role of vitamin D in improving cognitive dysfunction caused by sevoflurane anesthesia in aged mice

作　　者：张佳雷 武杰 杨永妍 于泳浩[2] Zhang Jialei;Wu Jie;Yang Yongyan;Yu Yonghao(Department of Pain Medicine,Changzhi People's Hospital Affiliated to Changzhi Medical College,Changzhi 046000,China;Depart-ment of Anesthesiology,Tianjin Medical University General Hospital,Tianjin 300052,China)

机构地区：[1]长治医学院附属长治市人民医院疼痛科,长治046000 [2]天津医科大学总医院麻醉科,天津300052

出　　处：《国际麻醉学与复苏杂志》2024年第9期917-922,共6页International Journal of Anesthesiology and Resuscitation

基　　金：长治医学院附属长治市人民医院临床基础创新研究项目(2022A023)。

摘　　要：目的探讨维生素D(VD)在改善七氟醚麻醉致老年小鼠认知功能障碍中的作用。方法清洁级健康12月龄C57BL/6J小鼠40只,采用随机数字表法分为4组(每组10只):对照组(C组)、正常老年小鼠+七氟醚组(S组)、VD(+)老年小鼠+七氟醚组[VD(+)组]和VD(-)老年小鼠+七氟醚组[VD(-)组]。采用骨化三醇灌胃构建VD(+)老年小鼠,采用避光及特殊饲料饲养构建VD(-)老年小鼠。S组、VD(+)组、VD(-)组小鼠在密闭环境中每日吸入60%O2与3%七氟醚混合气体,连续3 d;C组小鼠每日吸入60%O2,连续3 d。模型建立成功后第3天进行连续6 d的水迷宫实验,记录4组小鼠逃避潜伏期(第1~5天)与穿越平台次数(第6天)。取小鼠脑海马组织,采用酶联免疫吸附测定法检测VD含量,羟胺法测定超氧化物歧化酶(SOD)活性,钼酸铵比色法测定过氧化物酶(CAT)活性,硫代巴比妥酸法测定丙二醛(MDA)含量,采用免疫印迹法(Western blot)测定突触素(SYN)、突触后致密蛋白-95(PSD-95)的表达情况。结果与C组比较:VD(+)组小鼠海马组织中VD含量较高(P<0.05),VD(-)组小鼠海马组织中VD含量则较低(P<0.05),S组小鼠海马组织中VD含量差异无统计学意义(P>0.05);S组与VD(-)组小鼠水迷宫实验中第2~5天逃避潜伏期较长(均P<0.05),穿越平台次数较少(均P<0.05);VD(+)组小鼠第2天逃避潜伏期较长(P<0.05),第3~5天逃避潜伏期和穿越平台次数差异无统计学意义(均P>0.05);S组、VD(+)组和VD(-)组小鼠海马组织中MDA含量较高(均P<0.05),CAT和SOD活性较低(均P<0.05),SYN和PSD-95表达均较低(均P<0.05)。与S组比较:VD(+)组小鼠第3~5天逃避潜伏期较短(均P<0.05),穿越平台次数较多(P<0.05),小鼠海马组织中MDA含量较低(P<0.05),CAT和SOD活性较高(均P<0.05),SYN和PSD-95表达较高(均P<0.05);VD(-)组小鼠第2~5天逃避潜伏期较长(均P<0.05),穿越平台次数较少(P<0.05),小鼠海马组织中MDA含量较高(P<0.05),CAT和SOD活性较低(均P<0.05),SYN和PSD-95表达�ObjectiveTo investigate the role of vitamin D(VD)in improving cognitive dysfunction caused by sevoflurane anesthesia in aged mice Methods A total of 40 clean-grade healthy 12-month-old C57BL/6J mice were utilized.According to the random number table method,they were divided into four groups(n=10):a control group(group C),a normal aged mice+sevoflurane group(group S),a VD(+)aged mice+sevoflurane group[group VD(+)]and a VD(-)aged mice+sevoflurane group[group VD(-)].The VD(+)aged mice were established by gavage with osteotriol,while the VD(-)aged mice were constructed by feeding with special feeds,without light.Mice in groups S,VD(+),and VD(-)inhaled a mixture of 60%O,and 3%sevoflurane daily in an airtight environment for three consecutive days,while those in group C inhaled 60%O,daily for three consecutive days.On day 3 after successful modeling,the water maze test was conducted for six consecutive days.The escape latency(day 1 to day 5)and the number of platform crossings(day 6)were recorded for the four groups of mice.Mouse hippocampal tissues were taken,and the VD content was detected by enzyme-linked immunosorbent assay,the activity of superoxide dismutase(SOD)was measured by hydroxylamine method,the activity of catalase(CAT)was measured by sodium molybdate colorimetry,the content of malondialdehyde(MDA)was measured by thiobarbituric acid method,the expression of synaptophysin(SYN)and post-synaptic density-95(PSD-95)were measured by Western blot.Results Compared with group C:group VD(+)showed increased VD content in hippocampal tissues of mice(P<0.05),while decreased VD content was seen in group VD(-)(P<0.05);there was no statistical difference in VD content in group S(P>0.05);group S and group VD(-)presented prolonged escape latency on days 2 to 5(all P<0.05),with a reduced number of plaform crossings(all P<0.05);group VD(+)showed a prolonged escape latency on day 2(P<0.05),without statistical differences in escape latency and the number of platform crossings on days 3 to 5(all P>0.05);the S,VD(+)and VD(-)grou

关 键 词：维生素D 七氟醚 认知功能障碍 小鼠 

分 类 号：R614[医药卫生—麻醉学]