蒙医心身互动疗法治疗慢性失眠的全转录组研究探索  

Exploring the Transcriptomic Profile of Mongolian Medicine Mind-Body Interactive Therapy for the Treatment of Chronic Insomnia

作　　者：房君 纳贡毕力格 浩日勒 高瑞明 沙日耐 FANG Jun;Nagongbilige;Haorile;GAO Ruiming;Sharinai(Institute of Mind-Body Medicine,Inner Mongolia Academy of Mongolian Medicine,Hohhot,Inner Mongolia Autonomous Region,010010,China;Department of Mongolian Medicine Mind-Body Medicine,Inner Mongolia International Mongolian Medicine Hospital,Hohhot,Inner Mongolia Autonomous Region,010065,China)

机构地区：[1]内蒙古自治区中蒙医药研究院心身医学研究所,内蒙古呼和浩特010010 [2]内蒙古自治区国际蒙医医院蒙医心身医学科,内蒙古呼和浩特010065

出　　处：《中国民族医药杂志》2024年第9期65-70,共6页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：内蒙古自然科学基金,NO2018MS08011;中国民族医药学会科研项目,2022M1105-600202。

摘　　要：目的:本研究旨在通过全转录组测序探索蒙医心身互动疗法对失眠患者的作用机制。方法:对5名慢性失眠患者进行了蒙医心身互动疗法的干预治疗,并且采集了干预治疗前后的静脉血进行了全转录组的高通量测序,通过比较分析治疗前后转录组数据的变化试图为揭示该心理疗法对治疗慢性失眠的作用机制奠定良好基础。结果:通过阿森斯失眠量表,匹兹堡睡眠质量指数和失眠严重程度指数量表检测发现接受蒙医心身互动疗法治疗前和后的慢性失眠患者在睡眠方面有显著的临床改善(P≤0.05)。全转录组分析发现与蒙医心身互动疗法治疗前相比较,治疗后有838个差异表达mRNA,其中425个mRNA是下调的,413个mRNA是上调的;有2445个差异lncRNA,1211个lncRNA是下调的,1234个lncRNA是上调的;有29个差异表达的miRNA,其中包括12个上调miRNA和17个下调的miRNA。显著差异的mRNA显著富集在与细胞因子相关的通路上;进一步分析该通路上的差异基因的ceRNA,发现miRNA-lncRNA-mRNA三元网络包括了11个miRNA,23个lncRNA和23个mRNA节点。结论:通过本实验发现通过蒙医心身互动疗干预后慢性失眠患者的炎症因子IL17b降低,与之相关的LOC285889,COL18A1-AS,hsa-miR-1249-3p和hsa-miR-1908-5p也有显著改变,需要进一步扩大样本继续探索。Objective:The aim of this study is to investigate the underlying mechanisms of action of Mongolian Mind-Body Interactive Therapy(MMBIT)in patients with insomnia through whole transcriptome sequencing.Methods:A total of 5 patients with chronic insomnia were subjected to intervention treatment using MMBIT.Peripheral blood samples were collected before and after the intervention treatment for high-throughput transcriptome sequencing.By comparing and analyzing the changes in transcriptomic data before and after treatment,we aim to establish a solid foundation for uncovering the mechanisms of action of MMBIT in treating chronic insomnia.Results:Significant clinical improvements in sleep were observed in patients with chronic insomnia before and after receiving MMBIT,as assessed by the Athens Insomnia Scale,Pittsburgh Sleep Quality Index,and Insomnia Severity Index(P≤0.05).Whole transcriptome analysis revealed 838 differentially expressed mRNAs after treatment,with 425 downregulated and 413 upregulated mRNAs.Additionally,2,445 differentially expressed lncRNAs were identified,with 1,211 downregulated and 1,234 upregulated lncRNAs.Moreover,29 differentially expressed miRNAs were detected,including 12 upregulated miRNAs and 17 downregulated miRNAs.Significantly different mRNAs were found to be significantly enriched in pathways associated with cytokines.Further analysis of the differentially expressed genes in this pathway using the ceRNA network revealed an interaction network involving 11 miRNAs,23 lncRNAs,and 23 mRNA nodes.Conclusion:This study revealed that MMBIT intervention led to a reduction in the inflammatory cytokine IL17b in patients with chronic insomnia.Additionally,significant changes were observed in the expression of LOC285889,COL18A1-AS,hsa-miR-1249-3p,and hsamiR-1908-5p,which are associated with IL17b.Further investigation with an expanded sample size is warranted to explore these findings in greater detail.

关 键 词：蒙医心身互动疗法 慢性失眠 全转录组 三元网络 

分 类 号：R56.23[医药卫生—呼吸系统]