补肾泄浊方抑制NLRP3炎症小体激活改善高尿酸肾病肾脏组织炎症及纤维化  

Bushen Xiezhuo Formula improving inflammation and fibrosis of renal tissue in hyperuric acid nephropathy ameliorated by inhibiting NLRP3 inflammatory vesicle activation

作　　者：谷雨轩 张梦娇 盛冬云 赵晖 杨化冰 GU Yu-xuan;ZHANG Meng-jiao;SHEN Dong-yun;ZHAO Hui;YANG Hua-bing(Hubei University of Chinese Medicine,Wuhan 430065;Hubei Shizhen Laboratory,Wuhan 430065;Department of TCM,General Hospital of China Resources WISCO,Wuhan 430080)

机构地区：[1]湖北中医药大学基础医学院,湖北武汉430065 [2]湖北时珍实验室,湖北武汉430065 [3]华润武钢总医院中医科,湖北武汉430080

出　　处：《湖北中医药大学学报》2024年第5期5-9,共5页Journal of Hubei University of Chinese Medicine

基　　金：湖北省卫健委中医药科研项目(项目编号:ZY2021M107);湖北省教育厅2020年高校优秀中青年创新团队项目(项目编号:T2020013)。

摘　　要：目的观察补肾泄浊方抑制NLRP3炎症小体激活改善高尿酸肾病(HN)生理生化指标、肾脏炎症和纤维化的作用。方法32只SPF级雄性C57BL/6小鼠随机被分为正常组、模型组、补肾泄浊方组、四妙散组,每组8只。予以次腺嘌呤和氧嗪酸钾混悬液造模,同时予以补肾泄浊方、四妙散水煎液干预。结果补肾泄浊方能够改善HN小鼠的肾阳虚症状;对HN小鼠肾脏组织炎症及纤维化病变均有不同程度的改善作用;小鼠肾脏组织炎症相关因子表达降低,结论补肾泄浊方能够显著改善HN小鼠的肾阳虚症状及HN小鼠血常规生理生化指标,保护肾功能,显著改善小鼠肾脏炎症病变,抑制肾脏组织NLRP3炎症小体激活,减少促炎因子表达。Objective To observe the effect of Bushenxiezhuo Formula(BSXZF)to improve the physiological and biochemical indexes,renal inflammation and fibrosis in hyperuric acid nephropathy(HN).Methods Male C57BL/6 mice were randomly divided into normal group,model group,BSXZF group and the Simiaosan(SMS)group.Hypoadenine and potassium oxybate suspension was used for modelling,and BSXZF and Simiaosan aqueous decoction were used for intervention at the same time.Results BSXZF can improve the symptoms of renal yang deficiency in HN mice;it has different degrees of improvement on the inflammatory and fibrotic lesions in the kidney tissues of HN mice;and the expression of inflammationrelated factors in the kidney tissues of mice was reduced.Conclusion BSXZF can significantly improve the inflammatory lesions in the kidneys,inhibit the activation of NLRP3 inflammatory vesicles and reduce the expression of pro-inflammatory factors in the kidneys.It can significantly improve the renal fibrosis lesions in HN mice and reduce the expression of fibrosisrelated factors in renal tissues.

关 键 词：高尿酸血症肾病 补肾泄浊方 肾脏损伤 炎症 纤维化 

分 类 号：R285.5[医药卫生—中药学]