虫草保肾颗粒对肾性骨病大鼠的改善作用及Klotho/FGF-23通路的影响  

Improving effect of Chongcao Baoshen Granules on renal osteodystrophy rats and its impact on the Klotho/FGF-23 pathway

作　　者：赵俸玉 孙洋 刘娇 徐敢风 ZHAO Feng-yu;SUN Yang;LIU Jiao;XU Gan-feng(Heilongjiang Provincial Academy of Traditional Chinese Medicine,Harbin 150036)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036

出　　处：《湖北中医药大学学报》2024年第5期10-13,共4页Journal of Hubei University of Chinese Medicine

基　　金：黑龙江省中医药科研项目(项目编号:ZHY2020-040)。

摘　　要：目的观察虫草保肾颗粒(CCBSG)对肾性骨病(ROD)大鼠的改善作用及Klotho/成纤维细胞生长因子-23(FGF-23)通路的影响。方法将72只大鼠随机分为对照组、ROD组、CCBSG低剂量组、CCBSG中剂量组、CCBSG高剂量组、骨化三醇组,每组12只。除对照组外,其它组大鼠均需通过5/6肾切除联合高磷饲养的方式构建ROD大鼠模型,建模成功后,进行给药处理,给药1次/d,持续12周。自动生化分析仪检测大鼠血清中尿素氮(BUN)、血肌酐(Scr)、钙(Ca^(2+))、磷(P^(3+))水平;ELISA法检测大鼠血清中全段甲状旁腺素(iPTH)水平;双能X射线检测大鼠股骨骨密度;HE染色评估股骨组织病理变化;Western Blot检测Klotho、FGF-23蛋白表达。结果与对照组比较,ROD组大鼠股骨病理损伤严重,血清中BUN、Scr、P^(3+)、iPTH水平及股骨组织中FGF-23蛋白表达升高,Ca^(2+)水平、骨密度及股骨组织中Klotho蛋白表达下调(P<0.05);与ROD组比较,CCBSG低剂量组、CCBSG中剂量组、CCBSG高剂量组、骨化三醇组大鼠股骨病理损伤减轻,血清中BUN、Scr、P^(3+)、iPTH水平及股骨组织中FGF-23蛋白表达降低,Ca^(2+)水平、骨密度及股骨组织中Klotho蛋白表达升高(P<0.05)。结论CCBSG改善大鼠ROD可能与调控Klotho/FGF-23通路有关。Objective To observe the improvement effect of Chongcao Baoshen Granules(CCBSG)on renal osteodystrophy(ROD)rats and the influence on Klotho/fibroblast growth factor-23(FGF-23)pathway.Methods A total of 72 rats were randomly separated into control group,ROD group,low dose,medium dose,and high-dose CCBSG groups,and calcitriol group,12 in each group.Except control group,all other groups were used to construct ROD rat models through 5/6 nephrectomy combined with high phosphorus feeding,and after successful modeling,medication was administered once a day for 12 weeks.Automatic biochemical analyzer was applied to detect levels of urea nitrogen(BUN),creatinine(Scr),calcium(Ca^(2+)),and phosphorus(P^(3+))in serum;ELISA method was applied to detect the level of intact parathyroid hormone(iPTH)in serum;dual energy X-ray was applied to detect bone mineral density of femur;HE staining was used to evaluate the pathological changes of femoral tissue;Western Blot was used to detect Klotho and FGF-23 proteins expression in femoral tissue.Results Compared with control group,the pathological damage of the femur in ROD group was severe,the levels of BUN,Scr,P^(3+),iPTH in serum and the expression of FGF-23 protein in femoral tissue increased,the level of Ca^(2+),bone density,and Klotho protein expression in femoral tissue decreased(P<0.05);compared with ROD group,the pathological damage to the femur of rats in low dose,medium dose,and high dose CCBSG groups,and calcitriol group was alleviated,the levels of BUN,Scr,P^(3+),iPTH in serum and the expression of FGF-23 protein in femoral tissue decreased,the level of Ca^(2+),bone density,and the protein expression of Klotho in femoral tissue increased(P<0.05).Conclusion The improvement of rat ROD by CCBSG may be related to the regulation of the Klotho/FGF-23 pathway.

关 键 词：肾性骨病 虫草保肾颗粒 Klotho/成纤维细胞生长因子-23通路 股骨 

分 类 号：R285.5[医药卫生—中药学]