Efficient control of root-knot nematodes by expressing Bt nematicidal proteins in root leucoplasts  

作　　者：Yong Wang Mengnan Wang Yali Zhang Longwei Peng Dadong Dai Fengjuan Zhang Jiang Zhang 

机构地区：[1]State Key Laboratory of Biocatalysis and Enzyme Engineering,Hubei Hongshan Laboratory,School of Life Sciences,Hubei University,Wuhan 430062,China [2]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518000,China [3]State Key Laboratory of Agricultural Microbiology,College of Life Science and Technology,Huazhong Agricultural University,Wuhan,China

出　　处：《Molecular Plant》2024年第10期1504-1519,共16页分子植物（英文版）

基　　金：supported by the National Natural Science Foundation of ChinaNational Natural Science Foundation of China(32271546);the Innovation Program of Chinese Academy of Agriculture Sciences(CAAS-CSCB-202402);the Natural Science Foundation of Hubei ProvinceNatural Science Foundation of Hubei Province(2022CFB482).

摘　　要：Root-knot nematodes(RKNs)are plant pests that infect the roots of host plants.Bacillus thuringiensis(Bt)nematicidal proteins exhibited toxicity to nematodes.However,the application of nematicidal proteins for plant protection is hampered by the lack of effective delivery systems in transgenic plants.In this study,we discovered the accumulation of leucoplasts(root plastids)in galls and RKN-induced giant cells.RKN infection causes the degradation of leucoplasts into small vesicle-like structures,which are responsible for delivering proteins to RKNs,as observed through confocal microscopy and immunoelectron microscopy.We showed that different-sized proteins from leucoplasts could be taken up by Meloidogyne incognita female.To further explore the potential applications of leucoplasts,we introduced the Bt crystal protein Cry5Ba2 into tobacco and tomato leucoplasts by fusing it with a transit peptide.The transgenic plants showed significant resistance to RKNs.Intriguingly,RKN females preferentially took up Cry5Ba2 protein when delivered through plastids rather than the cytosol.The decrease in progeny was positively correlated with the delivery efficiency of the nematicidal protein.In conclusion,this study offers new insights into the feeding behavior of RKNs and their ability to ingest leucoplast proteins,and demonstrates that root leucoplasts can be used for delivering nematicidal proteins,thereby offering a promising approach for nematode control.

关 键 词：ROOT leucoplast Bacillus thuringiensis Cry5Ba2 protein root-knot nematodes pest control 

分 类 号：Q94[生物学—植物学]