Natural variations of maize ZmLecRK1 determine its interaction with ZmBAK1 and resistance patterns to multiple pathogens  

作　　者：Zhenju Li Junbin Chen Chuang Liu Shengfeng He Mingyu Wang Lei Wang Vijai Bhadauria Shiwei Wang Wenyu Cheng Hui Liu Xiaohong Yang Mingliang Xu You-Liang Peng Wangsheng Zhu 

机构地区：[1]State Key Laboratory of Maize Bio-breeding/College of Plant Protection/Ministry of Agriculture and Rural Affairs Key Laboratory of Surveillance and Management for Plant Quarantine Pests,China Agricultural University,Beijing 100193,P.R.China [2]Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Zhejiang 310021,P.R.China [3]Yazhouwan National Laboratory,Sanya,Hainan 572024,P.R.China [4]Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control,College of Plant Protection,China Agricultural University,Beijing 100193,P.R.China [5]State Key Laboratory of Plant Environmental Resilience,College of Agronomy and Biotechnology,National Maize Improvement Center,Center for Crop Functional Genomics and Molecular Breeding,China Agricultural University,Beijing 100193,P.R.China

出　　处：《Molecular Plant》2024年第10期1606-1623,共18页分子植物（英文版）

基　　金：supported by Biological Breeding-National Science and Technology Major Project(no.2023ZD04070,W.Z.);the National Key Research and Development Program,Ministry of Science and Technology of China(no.2022YFD1201802,W.Z.);the National Natural Science Foundation of China(no.32472499,W.Z.);the Pinduoduo-China Agricultural University Research Fund(no.PC2023A01005,Y.-L.P.).

摘　　要：Maize(Zea mays)is one of the most important crops in the world,but its yield and quality are seriously affected by diverse diseases.Identifying broad-spectrum resistance genes is crucial for developing effective strategies to control the disease in maize.In a genome-wide study in maize,we identified a G-type lectin receptor kinase ZmLecRK1,as a new resistance protein against Pythium aphanidermatum,one of the causal pathogens of stalk rot in maize.Genetic analysis showed that the specific ZmLecRK1 allele can confer resistance to multiple pathogens in maize.The cell death and disease resistance phenotype mediated by the resistant variant of ZmLecRK1 requires the co-receptor ZmBAK1.A naturally occurring A404S variant in the extracellular domain of ZmLecRK1 determines the ZmLecRK1-ZmBAK1 interaction and the formation of ZmLecRK1-related protein complexes.Interestingly,the ZmLecRK1 susceptible variant was found to possess the amino acid S404 that is present in the ancestral variants of ZmLecRK1 and conserved among the majority of grass species,while the resistance variant of ZmLecRK1 with A404 is only present in a few maize inbred lines.Substitution of S by A at position 404 in ZmLecRK1-like proteins of sorghum and rice greatly enhances their ability to induce cell death.Further transcriptomic analysis reveals that ZmLecRK1 likely regulates gene expression related to the pathways in cell wall organization or biogenesis in response to pathogen infection.Taken together,these results suggest that the ZmLecRK1 resistance variant enhances its binding affinity to the co-receptor ZmBAK1,thereby enhancing the formation of active complexes for defense in maize.Our work highlights the biotechnological potential for generating disease-resistant crops by precisely modulating the activity of ZmLecRK1 and its homologs through targeted base editing.

关 键 词：natural variation G-type lectin receptor-like kinase co-receptor BAK1 broad-spectrum resistance genome-wide association study MAIZE 

分 类 号：S513[农业科学—作物学]