DNA链置换反应在可视化键盘锁安全系统中的应用  

Application of DNA Strand Displacement Reaction in the Visual Keypad Lock Security System

作　　者：赵鑫月 殷志祥 刘娟[1] ZHAO Xinyue;YIN Zhixiang;LIU Juan(School of Mathematics and Physics,Bengbu University,Bengbu 233000,China;School of Mathematics and Statistics,Shanghai University of Engineering Science,Shanghai 201620,China)

机构地区：[1]蚌埠学院数理学院,安徽蚌埠233000 [2]上海工程技术大学数理与统计学院,上海201620

出　　处：《宿州学院学报》2024年第9期17-21,共5页Journal of Suzhou University

基　　金：国家自然科学基金资助项目(62072296,61672001);安徽省科学研究重点项目(KJ2020A0751);蚌埠学院科学研究项目(2020ZR15);蚌埠学院教育教学改革研究项目(2022jyxm18)。

摘　　要：利用DNA链置换反应设计了输入模块、反应模块和检测模块等三个模块。输入模块,将不同顺序的单链加入反应模块中;反应模块,只有输入正确顺序的DNA链才能完全与反应模块的组合底物发生链置换反应,置换出DNA单链;检测模块,设计两种链置换反应,利用阈值比较,确定输入正确顺序DNA链的次数。通过荧光检测判定是否解锁成功,实现结果的可视化。该系统采用模块化结构,检测方法简单直观,只有输入DNA链顺序和次数都正确,才能精准地完成键盘锁的解锁,增大了密钥空间,具有优异的稳定性与安全性,为构建更复杂的信息安全系统提供了可能性。In this paper,three modules are designed by using DNA strand replacement reaction:input module,reaction module and detection module.The input module can add single strands in different sequences to the reaction module.Only the DNA strand with the correct input sequence can preferentially undergo a strand displacement reaction with the combined substrate of the reaction module to replace a single strand of DNA.Two types of strand displacement reactions were designed for the detection module,and threshold comparison was used to determine the number of times the correct sequence of DNA strands was inputted.This study uses fluorescence detection to determine whether unlocking is successful,achieving visualization of the results.The system adopts a modular structure,and the detection method is simple and intuitive.Only when the input DNA strand order and frequency are correct,can the keyboard lock be accurately unlocked,increasing the key space and having excellent stability and security,providing the possibility of building more complex information security systems.

关 键 词：DNA链置换 可视化键盘锁 逻辑门 阈值比较 

分 类 号：T301.6[一般工业技术]