温胆汤对肥胖痰湿证大鼠糖脂代谢及PI3K/Akt信号通路的影响  

Effects of Wendan Decoction on glucolipid metabolism and PI3K/Akt signaling pathway in obese rats with phlegm and dampness syndrome

作　　者：喻松仁 刘彩玲 钟友宝 熊乃鑫 徐佳玲 刘春燕 王萍[3] 张洁 程绍民[3] YU Songren;LIU Cailing;ZHONG Youbao;XIONG Naixin;XU Jialing;LIU Chunyan;WANG Ping;ZHANG Jie;CHENG Shaomin(Experimental Animal Science and Technology Center,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Xinjian District Hospital of Traditional Chinese Medicine,Nanchang 330100,China;Traditional ChineseMedicine School,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学实验动物科技中心,南昌330004 [2]南昌市新建区中医院,南昌330100 [3]江西中医药大学中医学院,南昌330004

出　　处：《中华中医药杂志》2024年第10期5468-5473,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江西省自然科学基金项目(No.20212BAB206010);江西省教育厅科学技术研究项目(No.GJJ201212);国家自然科学基金项目(No.81960851);江西省中医药管理局中医证候基础重点研究室(No.赣中医药科教字[2022]8号);江西中医药大学中医体质-状态辨识健康管理研究团队(No.CXTD22016)。

摘　　要：目的:观察温胆汤对肥胖痰湿证大鼠糖脂代谢、磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路关键分子蛋白表达的影响,探讨温胆汤干预肥胖痰湿证改善糖脂代谢的内在机制。方法:将126只大鼠随机分成2组:空白组16只,造模组110只,空白组采用基础饲料喂养,造模组采用高脂饲料喂养,共8周,制作肥胖痰湿证动物模型。造模成功后,视体质量情况,按序淘汰体质量过轻者,遴选出40只肥胖大鼠,随机分为模型对照(MC)组、奥利司他(ORLI)组、温胆汤低(WDD-L)、中(WDD-M)、高剂量(WDD-H)组,每组8只;另在空白组大鼠中随机选取8只设为正常对照(NC)组。ORLI组(32.40 mg/kg)、WDD-L组、WDD-M组和WDD-H组[按4.45、8.90、17.80 g/kg剂量(以生药量计算)]给予灌胃,MC和NC组则均给予等量蒸馏水灌胃,每天1次,共6周。治疗结束后,检测并计算大鼠体质量、Lee’s指数、脂体比和肥胖率,采用全自动生化分析仪检测大鼠血总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及空腹血糖(FBG)水平;采用酶联免疫吸附测定法(ELISA)检测空腹胰岛素(FINS),并计算稳态模型胰岛素抵抗指数(HOMA-IR);采用Western Blot检测腹周脂肪细胞PI3K/Akt信号通路关键分子PI3K、PIP3、p-Akt、Akt蛋白表达。结果:与空白组比较,造模组体质量和Lee’s指数显著升高(P<0.01),肥胖率>20%,有形体肥胖,活动度降低,食欲降低,皮毛无光泽、蓬松,便溏,对外界反应能力降低,饮水量减少等痰湿证型症状,说明高脂饲料喂养可成功复制肥胖痰湿证大鼠模型。与NC组比较,MC组大鼠体质量、Lee’s指数、脂体比显著升高(P<0.05,P<0.01),血脂、FBG、FINS及HOMA-IR水平均显著改变(P<0.01),腹周脂肪细胞PI3K、PIP3、p-Akt、Akt蛋白表达显著降低(P<0.01)。与MC组比较,各用药组大鼠肥胖痰湿证型症状显著改善,大鼠体质量、脂体比显著降低(P<0.05,P<0.01),血脂�Objective:To observe the effects of Wendan Decoction on glucose and lipid metabolism and the expression of key molecules of phosphatidylinositol-3-kinase-protein kinase B(PI3K/Akt)signaling pathway in obese rats with phlegm dampness syndrome,and to explore the mechanism underlying the intervention of Wendan Decoction in obese rats with phlegm-dampness syndrome to improve glucose and lipid metabolism.Methods:A total of 126 rats were randomly divided into 2 groups(110 rats in the modeling group and 16 rats in the blank group),and the blank group was fed with basal diet while the modeling group was fed with high-fat diet for 8 weeks to create an animal model of obesity with phlegm-dampness syndrome.After successful modeling,the rats with too light weight were eliminated in order according to their body weight,40 obese rats were selected according to their body mass,and randomly divided into model control(MC)group,orlistat(ORLI)group,Wendan Decoction low dose(WDD-L)group,Wendan Decoction medium dose(WDD-M)group,and Wendan Decoction high dose(WDD-H)group,8 rats in each group;and 8 rats were randomly selected from the blank group to be set as the normal control(NC)group.ORLI group(32.40 mg/kg),WDD-L group(4.45 g/kg),WDD-M group(8.90 g/kg)and WDD-H group(17.80 g/kg)were given gavage by dose(in raw drug quantity),respectively,and equal amounts of distilled water were given to both MC and NC groups once daily for 6 weeks.After the treatment,samples were taken after anesthesia to detect and calculate body mass,Lee's index,lipid-body ratio and obesity rate,and blood total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)and fasting blood glucose(FBG)levels were measured by automatic biochemical analyzer;Fasting insulin(FINS)was measured by enzyme-linked immunosorbent assay(ELISA)and the steady-state model insulin resistance index(HOMA-IR)was calculated;Western Blot was used to detect the expression of PI3K,PIP3,p-Akt and Akt proteins,key molecules of PI3K

关 键 词：温胆汤 肥胖 痰湿证 糖脂代谢 磷脂酰肌醇3-激酶/蛋白激酶B信号通路 机制 胰岛素抵抗 动物模型 

分 类 号：R285.5[医药卫生—中药学]