生防真菌T1-3的筛选、鉴定及对灰葡萄孢菌的拮抗作用研究  

Screening and Identification of Biocontrol Strain T1-3 and Its Antagonistic Effects on Botrytis Cinerea

作　　者：王雪妍[1] 岳丹丹[1] 徐宏光 潘梦诗 陈登辉 WANG Xueyan;YUE Dandan;XU Hongguang;PAN Mengshi;CHEN Denghui(Institute of Biology Co.Ltd.,Henan Academy of Sciences,Zhengzhou 450000,China)

机构地区：[1]河南省科学院生物研究所有限责任公司,郑州450000

出　　处：《河南科学》2024年第11期1602-1608,共7页Henan Science

基　　金：河南省科学院省级特聘研究员项目(230505002);河南省科学院基本科研业务费项目(220605075);河南省科学院博士启动经费项目(231816036);河南省科学院创新创业团队(20230114);河南省科学院杰出青年人才培养项目(210405010);河南省科学院科技创新平台培育与提升项目(241005024)。

摘　　要：为筛选能拮抗灰葡萄孢菌的真菌菌株作为灰霉病生物防治的菌株资源储备,从番茄的根际土壤中筛选得到一株对灰葡萄孢菌具有拮抗作用的真菌,通过形态学和分子生物学对其进行了鉴定,并对其拮抗机制进行了研究.结果表明,菌株T1-3为一株长枝木霉(Trichoderma longibrachiatum),其生长速率较病原菌快,对病原菌的抑菌率为87.75%;其无菌发酵滤液对病原菌的抑制率为84.75%,另对病原菌孢子萌发抑制率为80.51%,具有明显的抑制作用;且菌株T1-3生长过程中能够产生果胶酶和淀粉酶.长枝木霉T1-3具有良好的生防潜力和应用前景.To screen fungal strains that can antagonize Botrytis cinerea as a resource reserve for its biological control,a fungus with antagonistic effects against Botrytis cinerea was isolated from the rhizosphere soil of tomatoes.This fungus was identified through morphological and molecular biological methods,and its antagonistic mechanisms against pathogenic bacteria were studied.The results indicated that strain T1-3 is Trichoderma longibrachiatum,with a growth rate faster than that of pathogenic bacteria and an antibacterial rate of 87.75%.The bacteriogenic inhibition rate of the sterile fermentation filtrate was 84.75%,showing a significant inhibitory effect on the germination of pathogenic spores,with an inhibition rate of 80.51%.Additionally,strain T1-3 can produce pectinase and amylase during growth.Trichoderma longibrachiatum T1-3 shows significant biocontrol potential and promising application prospects.

关 键 词：长枝木霉 筛选 鉴定 灰葡萄孢菌 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治]