Interfacing biosynthetic CdS with engineered Rhodopseudomonas palustris for efficient visible light-driven CO_(2)-CH_(4) conversion  

作　　者：Yu Zhang Yulei Qian Zhenye Tong Su Yan Xiaoyu Yong Yang-Chun Yong Jun Zhou 

机构地区：[1]Bioenergy Research Institute,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816,China [2]Biofuels Institute,Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,School of Emergency Management&School of Environment and Safety Engineering,Jiangsu University,Zhenjiang 212013,China

出　　处：《Frontiers of Chemical Science and Engineering》2024年第10期1-12,共12页化学科学与工程前沿（英文版）

基　　金：supported by the 2022 Carbon Dafeng and Carbon Neutral Science and Technology Innovation Special Fund in Jiangsu Province(Grant No.BK20220003);the National Natural Science Foundation of China(Grant No.32371538);the Special Funds for Jiangsu Provincial Science and technology plan(Grant No.BZ2022052);the Jiangsu Agriculture Science and Technology Innovation Fund(Grant No.CX(21)2015);the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(Grant No.XTD2204).

摘　　要：Engineered photosynthetic bacterium Rhodo-pseudomonas palustris is excellent at one-step CO_(2) biomethanation and can use near-infrared light sources,overcoming the limitations of conventional photosynthetic systems.The current study constructed a biohybrid system that deposited CdS nanoparticles on R.palustris.This biohybrid system broadens the capture of sustainable solar energy,achieving a 155 nmol-mL-biological CH,production under full visible light irradiation,13.4-fold of that by the pure R.palustris.The transcriptome profiles revealed that gene expression related to photosynthetic electron transfer chain,nitrogenase,nanofilaments,and redox stress defense was activated.Accordingly,we attributed the much-enhanced CO_(2) biomethanation in the biohybrid system to the remarkable increase in the intracellular reducing power and the stronger rigidity of the cells assisted by photoexcited electrons from CdS nanoparticles.Our discovery offers insight and a promising strategy forimproving the current CO_(2)-CH_(4) biomanufacturing system.

关 键 词：CO_(2)methanation Rhodopseudomonas palustris CdS nanoparticles green catalysis 

分 类 号：TK6[动力工程及工程热物理—生物能] O643.36[理学—物理化学] TQ243.1[理学—化学]