兼抗4种烟草病毒病弱毒疫苗的构建与效果分析  

Construction and Efficacy Analysis of Attenuated Vaccine Against Four Types of Tobacco Virus Diseases

作　　者：于成明[1] 牟金泽 刘志菲 宋扬 陈呈涛 刘宏 魏学军 禚照卿 原雪峰[1] YU Chengming;MOU Jinze;LIU Zhifei;SONG Yang;CHEN Chengtao;LIU Hong;WEI Xuejun;ZHUO Zhaoqing;YUAN Xuefeng(College of Plant Protection,Shandong Agricultural University,Taian 271018,Shandong,China;People’s Government of Tianbao Town,Taian City,Taian 271211,Shandong,China;Gaomi Branch of Weifang Tobacco Company,Weifang 261500,Shandong,China)

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]山东省泰安市天宝镇人民政府,山东泰安271211 [3]山东潍坊市烟草有限公司高密分公司,山东潍坊261500

出　　处：《中国烟草科学》2024年第5期49-57,共9页Chinese Tobacco Science

基　　金：山东省现代农业技术体系资金资助项目(SDAIT-25-12);国家自然科学基金项目(32072382,32370174);烟草行业烟草病虫害监测与综合治理重点实验室开放课题(KLTPMIMT2022-16);山东潍坊烟草有限公司科技计划项目[潍烟技(2020)1号2020-26]。

摘　　要：为解决单靶标疫苗无法同时防治多种烟草病毒病的现状,以黄瓜花叶病毒Fny株系(Cucumber mosaic virus Fny,CMV_(Fny))为疫苗载体,利用重叠PCR技术在CMV_(Fny) RNA2弱毒突变体基础上以不同排列顺序插入TMV、PVY和TVBMV的部分基因组保守片段,构建了6种在本氏烟中交叉保护效果好、遗传稳定,并且对CMV、TMV、PVY和TVBMV 4种病毒均具有一定抗性的重组型病毒。6种重组型病毒对CMV单独侵染的防效均超过87%,对其他3种病毒单独侵染的防效为22.2%~46.2%,对4种病毒混合侵染的防效维持在16%~37%。本研究为烟草病毒病的防治提供了疫苗材料,丰富了植物病毒弱毒疫苗创制的实践。To address the challenge of a single-dose,single-target vaccine being unable control multiple tobacco viral diseases simultaneously,we constructed six recombinant viruses that exhibit strong cross-protection effective,stably existence in Nicotiana benthamiana and simultaneously resistance to CMV,TMV,PVY and TVBMV.These recombinant viruses all used the cucumber mosaic virus Fny(CMV_(Fny))strain as the vaccine vector,with conserved combination fragments of TMV,PVY and TVBMV genomes inserted into the CMV_(Fny) RNA2 attenuated mutant in different permutations.The relative control efficacy of the six recombinant viruses exceeded 87%against CMV infecting alone,ranging from 22.2%to 46.2%against single infections of the other three viruses,and from 16%to 37%against mixed infections of all four viruses.This study provides vaccine candidates for controlling tobacco virus diseases and enriches the practice of creating attenuated plant virus vaccines.

关 键 词：黄瓜花叶病毒 弱毒疫苗 交叉保护 多价疫苗 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]