烟草瞬时表达技术的多途径应用研究进展  

Advances and Prospects of Research on Multi-Pathway Applications of Tobacco Transient Expression Technology

作　　者：吴荣荣 汪阳忠 潘晓璐 倪琪 杨亚仑 郭永峰[1] 张增林[1] WU Rongrong;WANG Yangzhong;PAN Xiaolu;NI Qi;YANG Yalun;GUO Yongfeng;ZHANG Zenglin(Institute of Tobacco Research of CAAS,Municipal Key Laboratory of Plant Molecular Pharming,Qingdao 266101,China;Technical Center,Shanghai Tobacco Group Co.,Ltd.,Shanghai 201315,China)

机构地区：[1]中国农业科学院烟草研究所,青岛市植物分子医药重点实验室,青岛266101 [2]上海烟草集团有限责任公司技术中心,上海201315

出　　处：《中国烟草科学》2024年第5期114-120,共7页Chinese Tobacco Science

基　　金：中国烟草总公司重点研发项目(110202102026);山东省重点研发计划(科技示范工程)项目(2022SFGC0102)。

摘　　要：烟草瞬时表达是通过农杆菌介导的方式将目标基因导入烟草,短时间内获得目的重组蛋白质的现代生物工程技术。相较于传统的微生物发酵和动物细胞表达体系,烟草瞬时表达系统具有生产周期短、产量高、成本相对较低以及安全性好等优势。本文综述了烟草瞬时表达技术的发展历程,梳理了该技术在农业、工业和生物医药等领域的多元化应用现状,以及植物工厂和智慧农业对烟草瞬时表达技术产业化的支撑作用。同时,对于当前烟草瞬时表达技术向产业化迈进过程中面临的关键问题进行了探讨,提出了针对性的解决方案和策略建议,旨在为烟草瞬时表达技术的产业化应用提供参考。Tobacco transient expression is a cutting-edge bioengineering technology that rapidly introduces target genes into tobacco plants through Agrobacterium-mediated methods,enabling to obtain the target recombinant proteins in a short timeframe.Compared to traditional microbial fermentation and animal cell expression systems,the tobacco transient expression system offers several the advantages,including short production cycle,high yield,cost-effectiveness and enhanced safety.This paper reviews the historical development of tobacco transient expression technology,explores its diverse applications in agriculture,industry,and biomedicine,and highlights the supportive role of plant factories and smart agriculture in the industrialization of this technology.Additionally,it addresses the key challenges currently hindering the industrialization of tobacco transient expression technology and proposes targeted solutions and strategies,with the goal of providing valuable insights for advancing its industrial application.

关 键 词：烟草 瞬时表达技术 研究进展 技术瓶颈 展望 

分 类 号：S572[农业科学—烟草工业] Q819[农业科学—作物学]