四丁基溴化膦存在下基于金纳米颗粒抗聚集的Cr^(3+)比色检测  

Colorimetric detection of Cr^(3+)based on anti-aggregation of gold nanoparticles in the presence of tetrabutylphosphonium bromide

作　　者：张克辉 饶红红[1] 罗明月 李建颖 刘海霞[1] 薛中华[2] ZHANG Ke-hui;RAO Hong-hong;LUO Ming-yue;LI Jian-ying;LIU Hai-xia;XUE Zhong-hua(School of Chemical Engineering,Lanzhou City University,Lanzhou 730070,Gansu,China;College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou 730070,Gansu,China)

机构地区：[1]兰州城市学院化学工程学院,甘肃兰州730070 [2]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2024年第6期78-86,I0003,共10页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省科技计划项目(21JR7RA538);甘肃省高校产业支撑计划项目(2023CYZC-69)。

摘　　要：探索简单、快速、便捷的铬离子(Cr^(3+))即时检测方法对于生态环境保护和人体健康监测具有重要意义.文中以无配体修饰的金纳米颗粒(Au NPs)为传感探针,通过目标物触发Au NPs的抗聚集效应,构建了一种简单、快速的Cr^(3+)比色检测方法.由于裸Au NPs表面缺乏配体保护,导致四丁基溴化膦(TPB)可以快速诱导其发生聚集,产生酒红到灰蓝的溶液颜色变化.然而,Cr^(3+)在较强的碱性条件下会形成高负电荷的多羟基配合物[Cr(OH)_4]^(-),并通过静电作用与TPB结合在一起,对裸Au NPs的聚集产生显著的抑制效应.这一机制使得Cr^(3+)对TPB/裸Au NPs聚集体系的抗聚集作用产生明显的溶液颜色变化,进而利用Au NPs体系的颜色信号对Cr^(3+)进行比色分析检测.结果表明,该方法不仅避免了繁琐的Au NPs表面修饰,而且具有较快的检测速度(<10s),对Cr^(3+)的检测限(LOD)可以达到1.1μmol·L~(-1),在即时检测(POCT)中展现出显著的应用潜力.Exploring simple,convenient,and rapid chromium ions(Cr^(3+)ions)point-of-care testing(POCT)method is important for environment and human health.Herein,a simple and rapid colorimetric detection method for Cr^(3+)was constructed by using ligand-free modified gold nanoparticles(i.e.,bare AuNPs)with target triggered anti-aggregation effect as a sensing probe.Since the surface of bare AuNPs lacked ligand protection,tetrabutylphosphonium bromide(TPB)can rapidly induce bare AuNPs aggregated,producing a wine-red to gray-blue solution color change.However,Cr^(3+)ions form highly negatively charged polyhydroxylated compounds[Cr(OH)4]^(-)in stronger alkaline conditions and bind to TPB via electrostatic interactions to inhibit the aggregation of bare AuNPs.Based on this mechanism,the anti-aggregation effect of Cr^(3+)ions on the TPB/bare AuNPs aggregation system can be translated into the colorimetric signal,and then the colorimetric detection of Cr^(3+)ions can be achieved by using the solution color change.This sensing method not only avoids the cumbersome surface modification of AuNPs,but also has a faster detection speed(<10 s).The LOD of Cr^(3+)ions is as low as 1.1μmol·L-1,which shows a strong potential for application in POCT.

关 键 词：比色传感 金纳米颗粒 抗聚集 铬离子 

分 类 号：O657.32[理学—分析化学]