150 g/L毒莠定·225 g/L二氯吡啶酸可溶液剂的高效液相色谱定量方法  

HPLC Method for Quantification of Picloram 150 g/L+Clopyralid 225 g/L Soluble Concentrate

作　　者：周清滕 吴艳青 周易安 刘志伟 胡贝贝 Zhou Qingteng;Wu Yanqing;Zhou Yian;Liu Zhiwei;Hu Beibei(Zhejiang Xinan Chemical Industrial Group Co.,LTD.,Hangzhou Zhejiang 311300,China)

机构地区：[1]浙江新安化工集团股份有限公司,浙江杭州311300

出　　处：《农药科学与管理》2024年第7期31-37,共7页Pesticide Science and Administration

摘　　要：建立了快速分析150 g/L毒莠定·225 g/L二氯吡啶酸可溶液剂复配产品的高效液相色谱(HPLC)定量方法。定量分析采用CAPCELLPAKC18(250 mm×4.6 mm×5μm)色谱柱,以乙腈/甲醇/磷酸水(pH 2.40)=20/10/70(V/V/V)作为流动相,在235 nm波长下对样品中的二氯吡啶酸和毒莠定进行分离测定。测定结果表明,二氯吡啶酸和毒莠定线性相关系数为0.9999、1.0000,变异系数为0.32%、0.63%,平均回收率为99.4%、100.6%,均满足定量控制要求。本文所提方法精密准确,分离良好,简捷稳定,可用于不同规格的单一制剂、复合制剂和原药中的二氯吡啶酸及毒莠定的定量分析。A high performance liquid chromatography(HPLC)method was developed for the rapid analysis of Picloram 150 g/L+Clopyralid 225 g/L Soluble Concentrate mixed formulation.The separation and determination of Clopyralid and Picloram in the samples were performed on a CAPCELL PAK C18(250 mm×4.6 mm×5μm)column with acetonitrile/methanol/phosphate water(pH 2.40)=20/10/70(V/V/V)as the mobile phase and by UV detector at 235 nm.The results showed that the linear correlation coefficient of Clopyralid and Picloram were 0.9999 and 1.0000,respectively,with coefficients of variation at 0.32%and 0.63%.The average recovery rates were 99.4%and 100.6%,meeting the requirements for quantitative control.The method developed in this paper is precise,accurate,simple and stable,and can be used for the quantitative analysis of Picloram and Clopyralid in single active ingredient preparations,mixed preparations,and technical materials under different specifications.

关 键 词：二氯吡啶酸 毒莠定 高效液相色谱 定量方法 

分 类 号：S482.4[农业科学—农药学] O657.72[农业科学—植物保护]