南极来源真菌OUCMDZ-4032的次级代谢产物研究  

Study of secondary metabolites of an Antarctic-derived fungus OUCMDZ-4032

作　　者：张庆庆 高海 张瑶 王乂[1,2] ZHANG Qingqing;GAO Hai;ZHANG Yao;WANG Yi(School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts,Qingdao Marine Science and Technology Center,Qingdao 266237,China)

机构地区：[1]中国海洋大学医药学院,山东青岛266003 [2]青岛海洋科技中心海洋药物与生物制品功能实验室,山东青岛266237

出　　处：《中国海洋药物》2024年第5期47-54,共8页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81973198,82111540166,U1906213)资助。

摘　　要：目的 对南极沉积物来源真菌OUCMDZ-4032的次级代谢产物进行研究。方法 运用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备高效液相色谱(HPLC)等方法对OUCMDZ-4032菌株的大米发酵提取物进行分离纯化;通过核磁共振谱(NMR)及质谱(MS)等波谱学方法进行结构解析并测定化合物的抗菌活性。结果 从真菌OUCMDZ-4032的大米发酵提取物中分离鉴定出8个化合物,其中2个二苯甲酮类化合物(1、2),1个嗜铁素类化合物(3),3个核苷类化合物(4、5、8),1个苯乙酸类化合物(6)和1个脂肪酸类的新化合物(7),其中化合物8为首次分离得到的天然产物。抗菌活性评价结果表明,化合物1~8对铜绿假单胞菌显示选择性抑制活性,最低抑菌浓度(MIC)为32μg/mL。Objective To investigate the secondary metabolites of an Antarctic sediment-derived fungus OUCMDZ-4032.Methods The fermentation extract of rice from OUCMDZ-4032 was purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography and semi-preparative high performance liquid chromatography(HPLC);the structure was determined by nuclear magnetic resonance spectroscopy(NMR) and mass spectrometry(MS).The antibacterial and antifungal activities were assayed.Results Eight compounds were isolated and identified from the extract of fungus OUCMDZ-4032,including two benzophenones(1,2),one ferredoxin(3),three nucleosides(4,5,8),one phenylacetate(6) and one new compound of fatty acid compound(7),of which compound 8 was isolated as a natural product for the first time.The results of antibacterial activity evaluation showed that compounds 1-8 had selective inhibitory activity against Pseudomonas aeruginosa with MIC value of 32 μg/mL.

关 键 词：南极真菌 次级代谢产物 抗菌活性 

分 类 号：R931.77[医药卫生—生药学]