基于肠道菌转化介导的桔梗总皂苷入血代谢产物鉴定研究  

Identify the metabolites of total saponins of Platycodonis Radix in blood based on intestinal bacteria-mediated method

作　　者：吴茜娃 张欣雨 钟渊涵 张雪梅 周瑜 冯艳 秦倩 张寿文 钟国跃 曾金祥 WU Xi-wa;ZHANG Xin-yu;ZHONG Yuan-han;ZHANG Xue-mei;ZHOU Yu;FENG Yan;QIN Qian;ZHANG Shou-wen;ZHONG Guo-yue;ZENG Jin-xiang(Research Center of Chinese Medicine Resources and Ethnic Minority Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;College of Pharmacy and Life Sciences,Jiujiang University,Jiujiang 332005,China)

机构地区：[1]江西中医药大学中药资源与民族药研究中心,江西南昌330004 [2]九江学院生命科学学院,江西九江332005

出　　处：《药学学报》2024年第11期3141-3152,共12页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金项目(82160736);江西中医药大学科技创新团队项目(CXTD22002)。

摘　　要：桔梗总皂苷入血产物的鉴定具有重要意义,但仍存在很大挑战。本研究采用超高效液相色谱-线性离子阱-静电场轨道阱高分辨质谱法(UPLC-LTQ-Orbitrap-MS),应用Compound Discoverer 3.2与Trace finder 2.1软件,首先从桔梗总皂苷中鉴定出8个类型共44个原形成分。在此基础上,以所鉴定的原形成分及其肠道菌转化逐级脱糖产物为分子模板构建肠道菌介导鉴定新方法,进一步从含药血浆中鉴定出来源于44个原形成分的57个入血产物。这些入血产物包括12个原形成分和45个代谢产物,且血中原形成分同时是其他原形成分的脱糖基代谢产物。本研究表明肠道菌介导新方法可有效鉴定桔梗总皂苷入血产物,揭示不同含量与口服生物利用度桔梗皂苷原形成分在血中的存在形式,并可阐明中药原形成分及其代谢产物的一对多与多对一网络。所有动物实验方案均获得江西中医药大学实验动物伦理委员会批准(编号:JZLLSC-202100322)。The identification of the components absorbed in serum of platycosides in total saponins fraction of Platycodonis Radix is great significance,but there are still great challenges.In this study,8 types of 44 primordial components from Platycodon saponins were firstly identified using the ultra-performance liquid chromatographylinear ion trap electrostatic field orbitrap high resolution mass spectrometry(UPLC-LTQ-Orbitrap-MS)equipped with the software of Compound Discoverer 3.2 and Trace finder 2.1.Then,the platycosides and their deglycosylated metabolites were used as the template molecules to construct the intestinal microbiota mediated method to identify the primary components and the prototypes in serum.As results,57 components originating from 44 prototypes of platycosides were identified from drug-containing plasma.Those compounds consist of 12 prototypes of platycosides and 45 metabolites,while the prototypes in serum are also deglycosylated metabolites of other platycosides.The results showed that the intestinal microbiota mediated method could be applied to identify the metabolites of platycosides in total saponins fraction of Platycodonis Radix;and reveal the potential existing forms of prototypes of platycosides in plasma;In addition,it could clearly illustrate the one to many and many to one network of the prototypes and metabolites of platycosides.All animal protocols were approved by the Animal Ethics Committee of Jiangxi University of Traditional Chinese Medicine(No.JZLLSC-202100322).

关 键 词：桔梗皂苷 原形成分 代谢产物 肠道菌介导方法 

分 类 号：R917[医药卫生—药物分析学]