艾叶水提取物对无乳链球菌和海豚链球菌的抗菌活性及其机制  

Antimicrobial activity and mechanism of water extract of Artemisia argyi against Streptococcus agalactiae and Streptococcus iniae

作　　者：朱海南 张志敏 武玉 王巧利 王一飞 ZHU Hainan;ZHANG Zhimin;WU Yu;WANG Qiaoli;WANG Yifei(Department of Cell Biology,College of Life Science and Technology,Jinan University,Guangzhou 510632,Guangdong,China;Tongbai County Tea and Mugwort Industry Development Center,Nanyang 474750,Henan,China)

机构地区：[1]暨南大学生命科学技术学院细胞生物学系,广东广州510632 [2]桐柏县茶艾产业发展中心,河南南阳474750

出　　处：《暨南大学学报（自然科学与医学版）》2024年第5期512-522,共11页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(82473972);广州市重点研发计划项目(202206010008)。

摘　　要：目的:从艾叶中筛选出一种抗无乳链球菌和海豚链球菌的提取物并探究其体外抗菌活性及机制。方法:通过琼脂平板扩散法筛选出体外抗菌效果优良的艾叶提取物,随后测定其对无乳链球菌和海豚链球菌的最低抑菌浓度,通过观测提取物处理后细菌生物被膜的形成、表面疏水性、相对电导率及菌体结构来探究其抗菌机制。结果:筛选出的抗菌效果良好的艾叶提取物为艾叶水提取物(Artemisia argyi water extract,AWE),其可通过抑制菌体生物被膜的形成、降低细菌表面疏水率、升高相对电导率而使细胞膜通透性增加,进而破坏细菌细胞膜和菌体结构。结论:AWE为抗菌效果优良的艾叶水提取物,具有作为无乳链球菌和海豚链球菌的天然抗菌剂的潜力。Objective:To screen an extract against Streptococcus agalactiae and Streptococcus iniae from Artemisia argyi Levl.et Vant,and to explore its in vitro antimicrobial activity and mechanism.Methods:The agar plate diffusion method was used to screen for Artemisia argyi Levl.et Vant extracts with excellent in vitro antibacterial effects.Subsequently,the minimum inhibitory concentration against Streptococcus agalactiae and Streptococcus iniae was determined.By observing the formation of biofilms,the hydrophobicity and relative conductivity of bacterial surfaces treated with the extract,and the structure of bacterial cells,its antibacterial mechanisms were explored.Results:The selected Artemisia argyi Levl.et Vant extract with good antibacterial effect was Artemisia argyi water extract(AWE).AWE can increase cell membrane permeability by inhibiting the formation of bacterial biofilm,reducing surface hydrophobicity,and increasing relative conductivity,thereby damaging bacterial cell membrane and bacterial structure.Conclusion:AWE is an excellent antibacterial extract of Artemisia argyi Levl.et Vant,which has the potential to serve as a natural antibacterial agent for Streptococcus agalactiae and Streptococcus iniae.

关 键 词：艾叶 无乳链球菌 海豚链球菌 抗菌 

分 类 号：R917[医药卫生—药物分析学]