水苏糖对腹泻儿童肠道菌群及代谢通路的影响  

Effects of stachyose on gut microbiota and metabolic pathways in children with diarrhea

作　　者：王兵 金慧[1] 皮雄娥[2] 吴彦[1] 王蔚然 刘伟[2] 赵刚[1] WANG Bing;JIN Hui;PI Xiong'e;WU Yan;WANG Weiran;LIU Wei;ZHAO Gang(Institute of Infectious Disease Control and Prevention,Hangzhou Center for Disease Control and Prevention,Hangzhou 310021,China;Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]杭州市疾病预防控制中心传染病防制所,杭州310021 [2]浙江省农业科学院植物保护与微生物研究所,杭州310021

出　　处：《中国公共卫生》2024年第9期1088-1094,共7页Chinese Journal of Public Health

基　　金：浙江省基础公益计划项目(LGN22C030008);杭州市农社发展科研重点项目(202004A20)。

摘　　要：目的利用宏基因组测序技术分析水苏糖对腹泻儿童肠道菌群的结构组成和代谢通路的影响。方法采集2021年12月—2022年3月某三甲医院收治的腹泻儿童粪便样本6份,利用体外模拟发酵技术接种于水苏糖培养基(STS组)和对照培养基(Ctrl组),提取粪便基因组DNA,通过宏基因组测序分析水苏糖对肠道微生物组成和多样性的影响,在COG和KEGG代谢功能层次上,PCoA分析肠道优势菌种在KEGG功能通路中的贡献度,分析代谢产物与肠道微生物、KEGG代谢通路之间的相关性。结果Alpha多样性分析结果显示,STS组与Ctrl组间除Simpson指数(0.29,0.24,P<0.05)差异有统计学意义外,Ace指数(143.17,127.00)、Chao指数(143.17,127.00)和Shannon指数(1.69,1.79)的差异均无统计学意义。STS组中长双歧杆菌(0.06%)、反刍木质杆菌(0.05%)和唾液乳酸杆菌(0.04%)的相对丰度高于Ctrl组。COG和KEGG代谢功能层次上的PCoA分析发现2组样本群落的差异有统计学意义(P<0.05)。STS组上调了嘌呤代谢、淀粉和蔗糖代谢、半乳糖代谢等特征性代谢通路,同时下调Ctrl组的特征性代谢通路。与Ctrl组相比,STS组中丰度增加的菌种在与KEGG level 3水平有关的代谢功能中表现出高贡献度。Spearman相关性系数分析发现肠道菌群的代谢产物与相关的肠道微生物、KEGG代谢通路之间存在密切联系。结论水苏糖可以调节腹泻儿童肠道菌群结构组成,增加肠道菌群多样性,促进相关的代谢功能,产生更多对人体有益的物质。Objective To analyze the effects of stachyose on the structural composition and metabolic pathways of gut microbiota in children with diarrhea using metagenomic sequencing technology.Methods Six stool samples were collected from children with diarrhea admitted to a tertiary hospital from December 2021 to March 2022 using the direct selection method.The samples were inoculated into stachyose medium(STS group)and control medium(Ctrl group)using in vitro simulated fermentation technology.Fecal genomic DNA was extracted,and the effects of stachyose on the composition and diversity of gut microbiota were analyzed through metagenomic sequencing.At the COG and KEGG metabolic function levels,PCoA analysis was performed to determine the contribution of dominant gut microbiota to KEGG functional pathways,and the correlations between metabolites and gut microbiota and KEGG metabolic pathways were analyzed.Results Alpha diversity analysis showed that except for the Simpson index(0.29,0.24,P<0.05),the differences in the Ace index(143.17,127.00),Chao index(143.17,127.00),and Shannon index(1.69,1.79)between the STS and Ctrl groups were not statistically significant.The relative abundances of Bifidobacterium longum(0.06%),Ruminococcus(0.05%),and Lactobacillus salivarius(0.04%)were higher in the STS group than in the Ctrl group.PCoA analysis at the COG and KEGG metabolic function levels revealed that the differences between the two groups were statistically significant(P<0.05).The STS group upregulated characteristic metabolic pathways such as purine metabolism,starch and sucrose metabolism,and galactose metabolism while downregulating the characteristic metabolic pathways of the Ctrl group.Compared with the Ctrl group,the increased abundance of microbiota in the STS group showed high contributions to metabolic functions related to KEGG level 3.Spearman's correlation coefficient analysis found close associations between metabolic products of gut microbiota and related gut microbiota and KEGG metabolic pathways.Conclusion Stachyos

关 键 词：水苏糖 腹泻 肠道菌群 代谢 宏基因组测序 

分 类 号：R725.7[医药卫生—儿科]