内芯外鞘神经导管的制备及表征  

Preparation and characterization of nerve guide conduit with core and sheath

作　　者：高欣 孔令驰 史安琪 钱运[2] 孙巍 游正伟[1] GAO Xin;KONG Lingchi;SHI Anqi;QIAN Yun;SUN Wei;YOU Zhengwei(College of Materials Science and Engineering,State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,Donghua University,Shanghai,China;Department of Orthopedics,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China)

机构地区：[1]东华大学材料科学与工程学院,纤维材料改性国家重点实验室,上海 [2]上海交通大学医学院附属上海市第六人民医院骨科,上海

出　　处：《东华大学学报（自然科学版）》2024年第6期30-36,共7页Journal of Donghua University(Natural Science)

基　　金：国家重点研发计划(2021YFC2400802);国家自然科学基金(52173117)。

摘　　要：神经导管在临床应用中存在单一材料力学性能不足、单一空管与神经结构不匹配、合成材料生物相容性差等问题,研发力学性能优良且具有仿生结构的神经导管成为新的发展方向。以水性聚氨酯(WPU)和丝素蛋白(SF)为原料,利用湿法纺丝和定向冷冻工艺构筑具有“内芯外鞘”结构的人工神经导管(A-NGC),并对其力学性能、微观结构、亲疏水性、细胞相容性等进行系统表征。结果表明:A-NGC具有明显的“芯鞘”结构,“外鞘”拉伸强度和韧性分别为(46.5±5.01)MPa和(116.4±13.69)MJ/m3,“内芯”以取向多孔片状结构规整排列,内外呈现差异化亲疏水界面(p<0.001),细胞相容性优异。大鼠步态分析结果证明A-NGC组的坐骨神经功能指数接近自体神经移植。研究可为开发新型仿生结构人工神经导管产品提供理论依据以及实践参考。Existing nerve guide conduits in clinical application have many shortcomings,including insufficient mechanical properties,structure mismatch with anatomy of natural nerve,and poor biocompatibility of synthetic materials.Nerve guide conduits with excellent mechanical properties and bionic structure have become a new development direction.An artificial nerve guide conduit(A-NGC)with the structure of inner core(topological silk fibroin)and outer sheath(waterborne polyurethane crosslinking network)was prepared by wet spinning and gradient freezing.Then,we systematically investigated the mechanical properties,microstructure,hydrophilicity and cytocompatibility of the A-NGC.The results show that the ANGC has an obvious“core-sheath”structure.The tensile strength and toughness of the“outer sheath”are(46.5±5.01)MPa and(116.4±13.69)MJ/m3,respectively,while the“inner core”is arranged in an orderly oriented porous sheet structure.The A-NGC exhibits differentiated hydrophilic/hydrophobic interfaces(p<0.001)and excellent cellular compatibility.Gait analysis demonstrates that the sciatic nerve function index of A-NGC group is close to the autologous nerve transplantation.The study provides theoretical basis and practical reference for the development of new artificial nerve guide conduit products.

关 键 词：神经导管 水性聚氨酯 丝素蛋白 取向结构 

分 类 号：TS101.4[轻工技术与工程—纺织工程]